NR1H4 Recombinant antibody
NR1H4 Recombinant Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human
Applications
WB, ELISA
Conjugate
Unconjugated
CloneNo.
4B4
验证数据展示
经过测试的应用
| Positive WB detected in | MKN-45 cells, HepG2 cells, HEK-293 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
81820-2-RR targets NR1H4 in WB, ELISA applications and shows reactivity with Human samples.
| Tested Applications | WB, ELISA |
| Tested Reactivity | Human |
| Immunogen | NR1H4 fusion protein Ag21948 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Full Name | nuclear receptor subfamily 1, group H, member 4 |
| Synonyms | BAR, Bile acid receptor, Farnesoid X activated receptor, Farnesol receptor HRR 1, FXR, HRR 1, HRR1, NR1H4, RIP14, RXR interacting protein 14 |
| Calculated Molecular Weight | 486 aa, 56 kDa |
| Observed Molecular Weight | 48 kDa |
| GenBank Accession Number | BC130573 |
| Gene Symbol | NR1H4 |
| Gene ID (NCBI) | 9971 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q96RI1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Nuclear Receptor subfamily 1, group H, member 4 (NR1H4, also known as FXR) is a receptor for bile acids and has an important role in regulating energy metabolism in liver, muscle and adipose tissues in humans and animals. NR1H4 is highly expressed in liver and has a role in lipid metabolism, whereas none of the other protein-coding genes in the region has known biological links with cholesterol, further supporting a role for NR1H4 in regulation of cholesterol levels. NR1H4 have four isoforms, i.e., FXRalpha1, FXRalpha2, FXRbeta1, and FXRbeta2, and each isoform has a different expressional level and transcriptional activity depending on its location within specific tissues. (PMID: 14733360, PMID: 30787420)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for NR1H4 antibody 81820-2-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |