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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated NR1H2 Monoclonal antibody

NR1H2 Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human, pig

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1E12B10

Cat No : CL488-60345

Print datasheet

Synonyms

Liver X receptor beta, LXR b, LXRB, NER, NER I, NR1H2, Nuclear receptor NER, Oxysterols receptor LXR beta, RIP15, UNR

验证数据展示

  • FC experiment of HepG2 using CL488-60345

    FC experiment of HepG2 using CL488-60345

    1X10^6 HepG2 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human NR1H2 (CL488-60345, Clone:1E12B10) (red), or 0.8 ug CoraLite® Plus 488 Mouse IgG1 Isotype Control (MOPC-21) (CL488-65124, Clone: MOPC-21) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive FC detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 60345-1-Ig

推荐稀释比

ApplicationDilution
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-60345 targets NR1H2 in FC (Intra) applications and shows reactivity with human, pig samples.

Tested Applications FC (Intra)
Tested Reactivity human, pig
Immunogen NR1H2 fusion protein Ag5635 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name nuclear receptor subfamily 1, group H, member 2
Synonyms Liver X receptor beta, LXR b, LXRB, NER, NER I, NR1H2, Nuclear receptor NER, Oxysterols receptor LXR beta, RIP15, UNR
Calculated Molecular Weight 51 kDa
Observed Molecular Weight 51 kDa
GenBank Accession NumberBC047750
Gene Symbol NR1H2
Gene ID (NCBI) 7376
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP55055
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NR1H2, also known as LXRB, is a liver X receptors, which form a subfamily of the nuclear receptor superfamily and are key regulators of macrophage function, controlling transcriptional programs involved in lipid homeostasis and inflammation. It preferentially bind to double-stranded oligonucleotide direct repeats, which have the consensus half-site sequence 5'-AGGTCA-3' and 4-nt spacing (DR-4). It involves in the regulation of cholesterol uptake through MYLIP-dependent ubiquitination of LDLR, VLDLR and LRP8.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 NR1H2 antibody CL488-60345Download protocol
Standard Protocols
Click here to view our Standard Protocols