验证数据展示
经过测试的应用
| Positive WB detected in | human liver tissue, pig liver tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
60345-1-Ig targets NR1H2 in WB, IHC, IF, ChIP, ELISA applications and shows reactivity with human, pig samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, ChIP |
| 经测试反应性 | human, pig |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag5635 Product name: Recombinant human NR1H2 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-461 aa of BC047750 Sequence: MSSPTTSSLDTPLPGNGPPQPGAPSSSPTVKEEGPEPWPGGPDPDVPGTDEASSACSTDWVIPDPEEEPERKRKKGPAPKMLGHELCRVCGDKASGFHYNVLSCEGCKGFFRRSVVRGGARRYACRGGGTCQMDAFMRRKCQQCRLRKCKEAGMREQCVLSEEQIRKKKIRKQQQQESQSQSQSPVGPQGSSSSASGPGASPGGSEAGSQGSGEGEGVQLTAAQELMIQQLVAAQLQCNKRSFSDQPKVTPWPLGADPQSRDARQQRFAHFTELAIISVQEIVDFAKQVPGFLQLGREDQIALLKASTIEIMLLETARRYNHETECITFLKDFTYSKDDFHRAGLQVEFINPIFEFSRAMRRLGLDDAEYALLIAINIFSADRPNVQEPGRVEALQQPYVEALLSYTRIKRPQDQLRFPRMLMKLVSLRTLSSVHSEQVFALRLQDKKLPPLLSEIWDVHE 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | nuclear receptor subfamily 1, group H, member 2 |
| 别名 | 1E12B10, Liver X receptor beta, LXR b, LXRB, NER |
| 计算分子量 | 51 kDa |
| 观测分子量 | 51 kDa |
| GenBank蛋白编号 | BC047750 |
| 基因名称 | NR1H2 |
| Gene ID (NCBI) | 7376 |
| RRID | AB_2881454 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | P55055 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
NR1H2, also known as LXRB, is a liver X receptors, which form a subfamily of the nuclear receptor superfamily and are key regulators of macrophage function, controlling transcriptional programs involved in lipid homeostasis and inflammation. It preferentially bind to double-stranded oligonucleotide direct repeats, which have the consensus half-site sequence 5'-AGGTCA-3' and 4-nt spacing (DR-4). It involves in the regulation of cholesterol uptake through MYLIP-dependent ubiquitination of LDLR, VLDLR and LRP8.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for NR1H2 antibody 60345-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun MARCH2 prevents doxorubicin-induced cardiomyopathy by stabilizing NR1H2 and promoting clearance of apoptotic cardiomyocytes. | ||
Nat Commun Oxysterol undersulfation promotes osteoarthritis by fueling chondrocyte lipid accumulation. | ||
Mol Cell mTOR inhibition reprograms cellular lipid homeostasis by inducing alternative lipid uptake and promoting cholesterol transport.
| ||
J Neuroinflammation Lysosomal cholesteryl ester hydrolysis drives white matter repair by reprogramming microglia into a novel reparative state. | ||
Acta Pharmacol Sin Liver X receptor agonists exert antitumor effects against hepatocellular carcinoma via inducing REPS2 expression | ||
Acta Pharmacol Sin Betaine-homocysteine methyltransferase protects against acetaminophen-induced acute liver failure via BACH1-SCD1-oleic acid axis |
