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验证数据展示

  • WB analysis using 85994-1-RR

    WB analysis using 85994-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85994-1-RR (NPTXR antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 85994-1-RR

    IHC staining of mouse brain using 85994-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 85994-1-RR (NPTXR antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 85994-1-RR

    IHC staining of mouse brain using 85994-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 85994-1-RR (NPTXR antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

NPTXR Recombinant antibody

NPTXR Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA
Cat No. 85994-1-RR

产品说明书

CloneNo. 250488B3

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

Neuronal pentraxin receptor

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inIMR-32 cells, mouse brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

85994-1-RR targets NPTXR in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原 NPTXR fusion protein Eg3109 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 neuronal pentraxin receptor
别名 Neuronal pentraxin receptor
计算分子量53 kDa
观测分子量35-65 kDa
GenBank蛋白编号NM_014293.3
基因名称 NPTXR
Gene ID (NCBI) 23467
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO95502
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for NPTXR antibody 85994-1-RRDownload protocol
IHC protocol for NPTXR antibody 85994-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols