验证数据展示

WB analysis using 10889-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10889-1-AP (NPTX2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
IF Staining of SH-SY5Y using 10889-1-AP
Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using NPTX2 antibody (10889-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).
IP experiment of mouse brain using 10889-1-AP
IP result of anti-NPTX2 (IP:10889-1-AP, 4ug; Detection:10889-1-AP 1:600) with mouse brain tissue lysate 4000ug.
IHC staining of human brain using 10889-1-AP
Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 10889-1-AP (NPTX2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human brain using 10889-1-AP
Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 10889-1-AP (NPTX2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
WB analysis using 10889-1-AP
SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 10889-1-AP (NPTX2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
IHC staining of rat brain using 10889-1-AP
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 10889-1-AP (NPTX2 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat brain using 10889-1-AP
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 10889-1-AP (NPTX2 antibody) at dilution of 1:800 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Featured Product
KD/KO Validated

NPTX2 Polyclonal antibody

NPTX2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 10889-1-AP

产品说明书

引用文献(23)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, ELISA and More (2)

别名

Neuronal pentraxin II, Neuronal pentraxin-2, NP2, NP-II

缓冲液配方: PBS, Azide, Glycerol

PBS, Azide, Glycerol

PBS Only

偶联物: Unconjugated

Unconjugated

规格	价格	库存

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概述

经过测试的应用
产品信息
发表文章 (23)
实验方案

产品说明书

MSDS

经过测试的应用

Positive WB detected in	mouse brain tissue, SH-SY5Y cells, rat brain tissue
Positive IP detected in	mouse brain tissue
Positive IHC detected in	human brain tissue, human pancreas cancer tissue, rat brain tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	SH-SY5Y cells

应用	推荐稀释比
Western Blot (WB)	WB : 1:1000-1:6000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

KD/KO	See 4 publications below
WB	See 13 publications below
IHC	See 8 publications below
IF	See 9 publications below
CoIP	See 1 publications below

产品信息

10889-1-AP targets NPTX2 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用	WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用	WB, IHC, IF, CoIP
经测试反应性	human, mouse, rat
文献引用反应性	human, mouse, rat
免疫原	CatNo: Ag1326 Product name: Recombinant human NPTX2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-354 aa of BC009924 Sequence: MLALLAASVALAVAAGAQDSPAPGSRFVCTALPPEAVHAGCPLPAMPMQGGAQSPEEELRAAVLQLRETVVQQKETLGAQREAIRELTGKLARCEGLAGGKARGAGATGKDTMGDLPRDPGHVVEQLSRSLQTLKDRLESLEHQLRANVSNAGLPGDFREVLQQRLGELERQLLRKVAELEDEKSLLHNETSAHRQKTESTLNALLQRVTELERGNSAFKSPDAFKVSLPLRTNYLYGKIKKTLPELYAFTICLWLRSSASPGIGTPFSYAVPGQANEIVLIEWGNNPIELLINDKVAQLPLFVSDGKWHHICVTWTTRDGMWEAFQDGEKLGTGENLAPWHPIKPGGVLILGQ 种属同源性预测
宿主/亚型	Rabbit / IgG
抗体类别	Polyclonal
产品类型	Antibody
全称	neuronal pentraxin II
别名	Neuronal pentraxin II, Neuronal pentraxin-2, NP2, NP-II
计算分子量	47 kDa
观测分子量	47-50 kDa, 55-60 kDa
GenBank蛋白编号	BC009924
基因名称	NPTX2
Gene ID (NCBI)	4885
RRID	AB_2153875
偶联类型	Unconjugated
形式	Liquid
纯化方式	Antigen affinity purification
UNIPROT ID	P47972
储存缓冲液	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

背景介绍

Neuronal pentraxins constitute a family of proteins that are homologous to C-reactive protein (CRP) and serum amyloid P component (SAP), including NPTX1, NPTX2, and the neuronal pentraxin receptor (NPTXR). NPTX2, also known as NARP (neuronal activity-regulated pentaxin), is a secreted protein involved in excitatory synapse formation. It also plays a role in the clustering of alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA)-type glutamate receptors at established synapses, resulting in non-apoptotic cell death of dopaminergic nerve cells. NPTX2 is highly up-regulated in Parkinson's disease (PD), suggesting it may be involved in the pathology of PD. (PMID: 12895424; 17987278; 10748068)

实验方案

Product Specific Protocols
IF protocol for NPTX2 antibody 10889-1-AP	Download protocol
IHC protocol for NPTX2 antibody 10889-1-AP	Download protocol
IP protocol for NPTX2 antibody 10889-1-AP	Download protocol
WB protocol for NPTX2 antibody 10889-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

发表文章

Species	Application	Title
human	IF	Nature A model of human neural networks reveals NPTX2 pathology in ALS and FTLD Authors - Marian Hruska-Plochan KD Validated View Article
mouse	IHC	Cell Res Disturbed engram network caused by NPTX downregulation underlies aging-related contextual fear memory deficits. Authors - Tao Jin KO Validated View Article
mouse	WB	Sci Transl Med The neuronal pentraxin Nptx2 regulates complement activity and restrains microglia-mediated synapse loss in neurodegeneration Authors - Jiechao Zhou KO Validated View Article
mouse	WB	Alzheimers Dement Reduced synaptic proteins and SNARE complexes in Down syndrome with Alzheimer's disease and the Dp16 mouse Down syndrome model: Impact of APP gene dose Authors - Xu-Qiao Chen View Article
human	CoIP	Cell Death Dis NPTX2 promotes colorectal cancer growth and liver metastasis by the activation of the canonical Wnt/β-catenin pathway via FZD6. Authors - Chunjie Xu View Article
human	WB	Mol Psychiatry Loss of schizophrenia risk gene XPO7 disrupts neuronal excitability and network regularity via altered Na(+) channel dynamics in human neurons. Authors - Lei Cui View Article

验证数据展示

WB analysis using 10889-1-AP

IF Staining of SH-SY5Y using 10889-1-AP

IP experiment of mouse brain using 10889-1-AP

IHC staining of human brain using 10889-1-AP

IHC staining of human brain using 10889-1-AP

WB analysis using 10889-1-AP

IHC staining of rat brain using 10889-1-AP

IHC staining of rat brain using 10889-1-AP

NPTX2 Polyclonal antibody

NPTX2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

经过测试的应用

推荐稀释比

发表文章中的应用

产品信息

背景介绍

实验方案

发表文章

A model of human neural networks reveals NPTX2 pathology in ALS and FTLD

Disturbed engram network caused by NPTX downregulation underlies aging-related contextual fear memory deficits.

The neuronal pentraxin Nptx2 regulates complement activity and restrains microglia-mediated synapse loss in neurodegeneration

Reduced synaptic proteins and SNARE complexes in Down syndrome with Alzheimer's disease and the Dp16 mouse Down syndrome model: Impact of APP gene dose

NPTX2 promotes colorectal cancer growth and liver metastasis by the activation of the canonical Wnt/β-catenin pathway via FZD6.

Loss of schizophrenia risk gene XPO7 disrupts neuronal excitability and network regularity via altered Na(+) channel dynamics in human neurons.

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