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  • KD/KO Validated

NPTX1 Polyclonal antibody

NPTX1 Polyclonal Antibody for IF/ICC,IF-P, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC,IF-P, IHC, WB, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 20656-1-AP

Print datasheet

Synonyms

Neuronal pentraxin 1, neuronal pentraxin I, NPTX1



经过测试的应用

Positive WB detected inmouse brain tissue, HepG2 cells, mouse liver tissue, mouse cerebellum tissue, rat cerebellum tissue
Positive IHC detected inmouse brain tissue, human gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inrat brain tissue, HeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20656-1-AP targets NPTX1 in WB, IF, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC,IF-P, IHC, WB, ELISA
Cited ApplicationsWB, IF, CoIP
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name neuronal pentraxin I
Synonyms Neuronal pentraxin 1, neuronal pentraxin I, NPTX1
Calculated Molecular Weight 47 kDa
Observed Molecular Weight 47 kDa
GenBank Accession NumberNM_002522
Gene Symbol NPTX1
Gene ID (NCBI) 4884
RRIDAB_10793669
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ15818
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NPTX1, also named as NP1, may mediate uptake of degraded synaptic material which could play an important role in synaptic remodeling. NPTX1 can mediate the neuronal and glial uptake of the snake venom toxin taipoxin. The antibody is specific to NPTX1.

实验方案

Product Specific Protocols
WB protocol for NPTX1 antibody 20656-1-APDownload protocol
IHC protocol for NPTX1 antibody 20656-1-APDownload protocol
IF protocol for NPTX1 antibody 20656-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB, CoIP

Front Immunol

Identification of Neuronal Pentraxins as Synaptic Binding Partners of C1q and the Involvement of NP1 in Synaptic Pruning in Adult Mice.

Authors - Réka Á Kovács
WB

Acta Biochim Biophys Sin (Shanghai)

miR-139-5p mediates the palmitate-induced inhibition of insulin secretion by targeting neuronal pentraxin 1 in INS-1 cells.

Authors - Kai Zhang
mouseIF

PLoS One

RNA-seq analysis of gene expression profiles in isolated stria vascularis from wild-type and Alport mice reveals key pathways underling Alport strial pathogenesis.

Authors - Brianna Dufek
humanWB

Cancer Manag Res

NPTX1 promotes metastasis via integrin/FAK signaling in gastric cancer.

Authors - Hongfei Yan
  • KD Validated
humanWB

PLoS One

Exosome-delivered circRPS5 inhibits the progression of melanoma via regulating the miR-151a/NPTX1 axis

Authors - Haijun Zhu
  • KD Validated
mouseWB

Oncogene

Chromatin-associated OGT promotes the malignant progression of hepatocellular carcinoma by activating ZNF263

Authors - Lingyan Wang