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验证数据展示

  • WB analysis using 26446-1-AP (same clone as 26446-1-PBS)

    WB analysis using 26446-1-AP (same clone as 26446-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 26446-1-AP (C16orf35 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 26446-1-PBS in a different storage buffer formulation.

  • IHC staining of human cervical cancer using 26446-1-AP (same clone as 26446-1-PBS)

    IHC staining of human cervical cancer using 26446-1-AP (same clone as 26446-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 26446-1-AP (NPRL3 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26446-1-PBS in a different storage buffer formulation.

  • IF Staining of A431 using 26446-1-AP (same clone as 26446-1-PBS)

    IF Staining of A431 using 26446-1-AP (same clone as 26446-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using NPRL3 antibody (26446-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 26446-1-PBS in a different storage buffer formulation.

NPRL3 Polyclonal antibody, PBS Only

NPRL3 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 26446-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

C16orf35, 14 gene protein, -14 gene protein, Alpha-globin regulatory element-containing gene protein, CGTHBA

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

26446-1-PBS targets NPRL3 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag24045

Product name: Recombinant human C16orf35 protein

Source: e coli.-derived, PET30a

Tag: 6*His

Domain: 1-390 aa of BC004185

Sequence: MADGNEGPQSPFHHILPKCKLARDLKEAYDSLCTSGVVRLHINSWLEVSFCLPHKIHYAASSLIPPEAIERSLKAIRPYHALLLLSDEKSLLGELPIDCSPALVRVIKTTSAVKNLQQLAQDADLALLQVFQLAAHLVYWGKAIIIYPLCENNVYMLSPNASVCLYSPLAEQFSHQFPSHDLPSVLAKFSLPVSLSEFRNPLAPAVQETQLIQMVVWMLQRRLLIQLHTYVCLMASPSEEEPRPREDDVPFTARVGGRSLSTPNALSFGSPTSSDDMTLTSPSMDNSSAELLPSGDSPLNQRMTENLLASLSEHERAAILSVPAAQNPEDLRMFARLLHYFRGRHHLEEIMYNENTRRSQLLMLFDKFRSVLVVTTHEDPVIAVFQALLP

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chromosome 16 open reading frame 35
别名 C16orf35, 14 gene protein, -14 gene protein, Alpha-globin regulatory element-containing gene protein, CGTHBA
计算分子量 64 kDa
观测分子量63 kDa
GenBank蛋白编号BC004185
基因名称 C16orf35
Gene ID (NCBI) 8131
RRIDAB_3742190
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ12980
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

NPRL3 (Nitrogen Permease Regulator Like 3) is a highly conserved tumor suppressor protein. As a core component of the GATOR1 complex, it serves as a key node in the amino acid sensing pathway and negatively regulates the mTORC1 signaling pathway, thereby controlling cell growth, proliferation, and autophagy. Loss of its function leads to hyperactivation of mTORC1 signaling. In neuroscience, loss-of-function mutations in NPRL3 are an important genetic cause of familial focal epilepsy, such as focal epilepsy with variable foci. In oncology, it is frequently inactivated through epigenetic silencing in various cancers, making it a significant tumor suppressor and a potential biomarker.

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