验证数据展示
经过测试的应用
| Positive WB detected in | K-562 cells, LNCaP cells, THP-1 cells |
| Positive IHC detected in | human cervical cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
26446-1-AP targets NPRL3 in WB, IHC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag24045 Product name: Recombinant human C16orf35 protein Source: e coli.-derived, PET30a Tag: 6*His Domain: 1-390 aa of BC004185 Sequence: MADGNEGPQSPFHHILPKCKLARDLKEAYDSLCTSGVVRLHINSWLEVSFCLPHKIHYAASSLIPPEAIERSLKAIRPYHALLLLSDEKSLLGELPIDCSPALVRVIKTTSAVKNLQQLAQDADLALLQVFQLAAHLVYWGKAIIIYPLCENNVYMLSPNASVCLYSPLAEQFSHQFPSHDLPSVLAKFSLPVSLSEFRNPLAPAVQETQLIQMVVWMLQRRLLIQLHTYVCLMASPSEEEPRPREDDVPFTARVGGRSLSTPNALSFGSPTSSDDMTLTSPSMDNSSAELLPSGDSPLNQRMTENLLASLSEHERAAILSVPAAQNPEDLRMFARLLHYFRGRHHLEEIMYNENTRRSQLLMLFDKFRSVLVVTTHEDPVIAVFQALLP 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | chromosome 16 open reading frame 35 |
| 别名 | C16orf35, 14 gene protein, -14 gene protein, Alpha-globin regulatory element-containing gene protein, CGTHBA |
| 计算分子量 | 64 kDa |
| 观测分子量 | 63 kDa |
| GenBank蛋白编号 | BC004185 |
| 基因名称 | C16orf35 |
| Gene ID (NCBI) | 8131 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q12980 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
NPRL3 (Nitrogen Permease Regulator Like 3) is a highly conserved tumor suppressor protein. As a core component of the GATOR1 complex, it serves as a key node in the amino acid sensing pathway and negatively regulates the mTORC1 signaling pathway, thereby controlling cell growth, proliferation, and autophagy. Loss of its function leads to hyperactivation of mTORC1 signaling. In neuroscience, loss-of-function mutations in NPRL3 are an important genetic cause of familial focal epilepsy, such as focal epilepsy with variable foci. In oncology, it is frequently inactivated through epigenetic silencing in various cancers, making it a significant tumor suppressor and a potential biomarker.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for NPRL3 antibody 26446-1-AP | Download protocol |
| WB protocol for NPRL3 antibody 26446-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
