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验证数据展示

  • WB analysis using 84043-1-RR

    WB analysis using 84043-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84043-1-RR (NPR1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 84043-1-RR

    IF Staining of HeLa using 84043-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NPR1 antibody (84043-1-RR, Clone: 240696A9 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

NPR1 Recombinant antibody

NPR1 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 84043-1-RR

产品说明书

CloneNo. 240696A9

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

GC A, ANPRA, ANPR A, ANPa, ANP A

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHT-29 cells, HeLa cells, SGC-7901 cells
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84043-1-RR targets NPR1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 natriuretic peptide receptor A/guanylate cyclase A (atrionatriuretic peptide receptor A)
别名 GC A, ANPRA, ANPR A, ANPa, ANP A
计算分子量 119 kDa
观测分子量 119-125 kDa
GenBank蛋白编号NM_000906
基因名称 NPR1
Gene ID (NCBI) 4881
RRIDAB_3671610
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP16066
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NPR1(Atrial natriuretic peptide receptor 1), also named as NPRA , is a transmembrane protein expressed in the vascular endothelium. Activation of NPRA requires a binding of natriuretic peptides NPPA (ANP) or NPPB (BNP) to its extracellular domain and this promotes the production of cyclic guanosine monophosphate (cGMP), which in turn activates protein kinase cGMP‐dependent 1 (PRKG1 or commonly used as PKG).

实验方案

Product Specific Protocols
WB protocol for NPR1 antibody 84043-1-RRDownload protocol
IF protocol for NPR1 antibody 84043-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols