验证数据展示
产品信息
29352-1-PBS targets NPPC in IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | IHC, IF-P, Indirect ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag29609 Product name: Recombinant human NPPC protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 74-126 aa of NM_024409 Sequence: DLRVDTKSRAAWARLLQEHPNARKYKGANKKGLSKGCFGLKLDRIGSMSGLGC 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | natriuretic peptide precursor C |
| 别名 | C type natriuretic peptide, CNP, CNP2 |
| 计算分子量 | 13 kDa |
| GenBank蛋白编号 | NM_024409 |
| 基因名称 | NPPC |
| Gene ID (NCBI) | 4880 |
| RRID | AB_3742310 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P23582 |
| 储存缓冲液 | PBS only, pH 7.3. |
| 储存条件 | Store at -80°C. The product is shipped with ice packs. Upon receipt, store it immediately at -80°C |
背景介绍
NPPC (C-type natriuretic peptide) is also named as CNP2, and belongs to the natriuretic peptide family. More recent studies have shown that in growing and preovulatory follicles, granulosa cells secrete into the follicular fluid, a C-type natriuretic peptide (NPPC, also known as CNP) capable of binding to cumulus cells membrane natriuretic peptide receptor 2 (NPR2, also known as guanylate cyclase B). The CNP/NPR2 complex, acting actively as guanylate cyclase, increases the concentration of cGMP both in cumulus cells and, indirectly, in the oocyte, maintaining meiosis arrest. The cAMP level is maintained due to the upstream activity type C natriuretic peptide (CNP, NPPC), produced by the granulosa cells, which is responsible for the production of cyclic guanosine monophosphate (cGMP) after binding to a specific NPR2 receptor (PMID: 35209923, PMID: 25183874, PMID: 20947764).
