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验证数据展示

  • WB analysis using 28085-1-AP (same clone as 28085-1-PBS)

    WB analysis using 28085-1-AP (same clone as 28085-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 28085-1-AP (NPC1L1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28085-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver using 28085-1-AP (same clone as 28085-1-PBS)

    IHC staining of human liver using 28085-1-AP (same clone as 28085-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 28085-1-AP (NPC1L1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28085-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver using 28085-1-AP (same clone as 28085-1-PBS)

    IHC staining of human liver using 28085-1-AP (same clone as 28085-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 28085-1-AP (NPC1L1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28085-1-PBS in a different storage buffer formulation.

NPC1L1 Polyclonal antibody, PBS Only

NPC1L1 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 28085-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

28085-1-PBS targets NPC1L1 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag26277

Product name: Recombinant human NPC1L1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 415-514 aa of NM_001101648

Sequence: MPNRSSYRYDSLLLGPKNFSGILDLDLLLELLELQERLRHLQVWSPEAQRNISLQDICYAPLNPDNTSLYDCCINSLLQYFQNNRTLLLLTANQTLMGQTS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 NPC1 (Niemann-Pick disease, type C1, gene)-like 1
别名
计算分子量 149 kDa
观测分子量140-149 kDa
GenBank蛋白编号NM_001101648
基因名称 NPC1L1
Gene ID (NCBI) 29881
RRIDAB_2918146
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UHC9
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

NPC1 like intracellular cholesterol transporter 1 (NPC1L1) is a multi-pass membrane protein which has 1332 amino acids and containing a conserved N-terminal Niemann-Pick C1 (NPC1) domain and a putative sterol-sensing domain (SSD). It is only found in the tubular membranes of primate hepatocytes and the brush-border membranes of the small intestine of mammals. PC1L1 is a key protein for intestinal absorption of cholesterol and plays an important role in cholesterol balance. Cholesterol contributes to cell proliferation, invasion and latency, and is essential for tumor formation and growth, so NPC1L1 plays a critical role in tumor development and spread (PMID: 36034854).

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