验证数据展示
经过测试的应用
| Positive WB detected in | THP-1 cells, RAW 264.7 cells, J774A.1 cells, C2C12 cells |
| Positive IF/ICC detected in | THP-1 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
88418-1-RR targets NOXA2/p67phox in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag8691 Product name: Recombinant human NCF2 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 179-526 aa of BC001606 Sequence: VGRLFRPNERQVAQLAKKDYLGKATVVASVVDQDSFSGFAPLQPQAAEPPPRPKTPEIFRALEGEAHRVLFGFVPETKEELQVMPGNIVFVLKKGNDNWATVMFNGQKGLVPCNYLEPVELRIHPQQQPQEESSPQSDIPAPPSSKAPGRPQLSPGQKQKEEPKEVKLSVPMPYTLKVHYKYTVVMKTQPGLPYSQVRDMVSKKLELRLEQTKLSYRPRDSNELVPLSEDSMKDAWGQVKNYCLTLWCENTVGDQGFPDEPKESEKADANNQTTEPQLKKGSQVEALFSYEATQPEDLEFQEGDIILVLSKVNEEWLEGECKGKVGIFPKVFVEDCATTDLESTRREV 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | neutrophil cytosolic factor 2 |
| 别名 | NCF2, NOXA2, P67PHOX, NCF 2, NCF-2 |
| 计算分子量 | 60 kDa |
| 观测分子量 | 67 kDa |
| GenBank蛋白编号 | BC001606 |
| 基因名称 | NCF2 |
| Gene ID (NCBI) | 4688 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P19878 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
NOXA2/p67phox is also named as NCF2(Neutrophil cytosol factor 2) and belongs to the NCF2/NOXA1 family. It is a membrane bound cytochrome b558 required for activation of the latent NADPH oxidase. NCF2 is an essential component of the multi-protein NADPH oxidase enzyme in phagocytic leukocytes, as well as in certain non-phagocytic cells. This protein has 4 isoforms produced by alternative splicing.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for NOXA2/p67phox antibody 88418-1-RR | Download protocol |
| WB protocol for NOXA2/p67phox antibody 88418-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
