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验证数据展示

  • WB analysis using 88418-1-RR (same clone as 88418-1-PBS)

    WB analysis using 88418-1-RR (same clone as 88418-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 88418-1-RR (NCF2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 88418-1-PBS in a different storage buffer formulation.

  • IF Staining of THP-1 using 88418-1-RR (same clone as 88418-1-PBS)

    IF Staining of THP-1 using 88418-1-RR (same clone as 88418-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using NOXA2/p67phox antibody (88418-1-RR, Clone: 260956B9 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 88418-1-PBS in a different storage buffer formulation.

NOXA2/p67phox Recombinant monoclonal antibody, PBS Only

NOXA2/p67phox Uni-rAb® Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 88418-1-PBS

产品说明书

CloneNo. 260956B9

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, Indirect ELISA

别名

NCF2, NOXA2, P67PHOX, NCF 2, NCF-2

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy NCF2 Ready-To-Use IHC Kit. NCF2 primary antibody included.

产品信息

88418-1-PBS targets NOXA2/p67phox in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag8691

Product name: Recombinant human NCF2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 179-526 aa of BC001606

Sequence: VGRLFRPNERQVAQLAKKDYLGKATVVASVVDQDSFSGFAPLQPQAAEPPPRPKTPEIFRALEGEAHRVLFGFVPETKEELQVMPGNIVFVLKKGNDNWATVMFNGQKGLVPCNYLEPVELRIHPQQQPQEESSPQSDIPAPPSSKAPGRPQLSPGQKQKEEPKEVKLSVPMPYTLKVHYKYTVVMKTQPGLPYSQVRDMVSKKLELRLEQTKLSYRPRDSNELVPLSEDSMKDAWGQVKNYCLTLWCENTVGDQGFPDEPKESEKADANNQTTEPQLKKGSQVEALFSYEATQPEDLEFQEGDIILVLSKVNEEWLEGECKGKVGIFPKVFVEDCATTDLESTRREV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 neutrophil cytosolic factor 2
别名 NCF2, NOXA2, P67PHOX, NCF 2, NCF-2
计算分子量 60 kDa
观测分子量 67 kDa
GenBank蛋白编号BC001606
基因名称 NCF2
Gene ID (NCBI) 4688
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP19878
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

NOXA2/p67phox is also named as NCF2(Neutrophil cytosol factor 2) and belongs to the NCF2/NOXA1 family. It is a membrane bound cytochrome b558 required for activation of the latent NADPH oxidase. NCF2 is an essential component of the multi-protein NADPH oxidase enzyme in phagocytic leukocytes, as well as in certain non-phagocytic cells. This protein has 4 isoforms produced by alternative splicing.

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