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CoraLite® Plus 488-conjugated NONO Polyclonal antibody
NONO Polyclonal Antibody for FC (Intra)
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
FC (Intra)
Conjugate
CoraLite® Plus 488 Fluorescent Dye
验证数据展示
经过测试的应用
| Positive FC detected in | HepG2 cells |
For other applications, we recommend the unconjugated version of this antibody, 11058-1-AP
推荐稀释比
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-11058 targets NONO in FC (Intra) applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | FC (Intra) |
| Tested Reactivity | human, mouse, rat |
| Immunogen | NONO fusion protein Ag1512 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | non-POU domain containing, octamer-binding |
| Synonyms | 55 kDa nuclear protein, NONO, NonO protein, NRB54, P54, p54(nrb), P54NRB |
| Calculated Molecular Weight | 54 kDa |
| Observed Molecular Weight | 54 kDa |
| GenBank Accession Number | BC010049 |
| Gene Symbol | NONO |
| Gene ID (NCBI) | 4841 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q15233 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Non-pou domain-containing octamer-binding protein(NONO), other named p54nrb, belongs to DBHS family, whose protein are predominately nuclear and involves in transcriptional and posttranscriptional gene regulatory functions as well as DNA repair. This family has roles in a wide range of biological processes, such as the regulation of circadian rhythm, carcinogenesis and progression of cancer. NONO forms heterodimer with SFPQ, and has been implicated in nuclear retention of A- to I-edited RNA as paraspeckle components, pre-mRAN 3'-end formation, cAMP cycling and transcriptional activation.
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 488 NONO antibody CL488-11058 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
