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CoraLite® Plus 488-conjugated NONO Polyclonal antibody

NONO Polyclonal Antibody for FC (Intra)
Cat No. CL488-11058

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

FC (Intra)

P54, NRB54, Non-POU domain-containing octamer-binding protein, NonO protein, 55 kDa nuclear protein

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -


经过测试的应用

Positive FC (Intra) detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 11058-1-AP

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-11058 targets NONO in FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原 NONO fusion protein Ag1512 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 non-POU domain containing, octamer-binding
别名 P54, NRB54, Non-POU domain-containing octamer-binding protein, NonO protein, 55 kDa nuclear protein
计算分子量 54 kDa
观测分子量 54 kDa
GenBank蛋白编号BC010049
基因名称 NONO
Gene ID (NCBI) 4841
RRIDAB_2923731
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ15233
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Non-pou domain-containing octamer-binding protein(NONO), other named p54nrb, belongs to DBHS family, whose protein are predominately nuclear and involves in transcriptional and posttranscriptional gene regulatory functions as well as DNA repair. This family has roles in a wide range of biological processes, such as the regulation of circadian rhythm, carcinogenesis and progression of cancer. NONO forms heterodimer with SFPQ, and has been implicated in nuclear retention of A- to I-edited RNA as paraspeckle components, pre-mRAN 3'-end formation, cAMP cycling and transcriptional activation.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 NONO antibody CL488-11058Download protocol
Standard Protocols
Click here to view our Standard Protocols
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