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验证数据展示

  • WB analysis using 15456-1-AP (same clone as 15456-1-PBS)

    WB analysis using 15456-1-AP (same clone as 15456-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 15456-1-AP (NOL12 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15456-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 15456-1-AP (same clone as 15456-1-PBS)

    WB analysis of HeLa using 15456-1-AP (same clone as 15456-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 15456-1-AP (NOL12 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15456-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 15456-1-AP (same clone as 15456-1-PBS)

    IP experiment of HeLa using 15456-1-AP (same clone as 15456-1-PBS)

    IP result of anti-NOL12 (IP:15456-1-AP, 4ug; Detection:15456-1-AP 1:300) with HeLa cells lysate 4000ug. This data was developed using the same antibody clone with 15456-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 15456-1-AP (same clone as 15456-1-PBS)

    IF Staining of HepG2 using 15456-1-AP (same clone as 15456-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NOL12 antibody (15456-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 15456-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

NOL12 Polyclonal antibody, PBS Only

NOL12 Polyclonal Antibody for WB, IF/ICC, IP, Indirect ELISA
Cat No. 15456-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, IP, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

15456-1-PBS targets NOL12 in WB, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag7723

Product name: Recombinant human NOL12 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-213 aa of BC002808

Sequence: MGRNKKKKRDGDDRRPRLVLSFDEEKRREYLTGFHKRKVERKKAAIEEIKQRLKEEQRKLREERHQEYLKMLAEREEALEEADELDRLVTAKTESVQYDHPNHTVTVTTISDLDLSGARLLGLTPPEGGAGDRSEEEASSTEKPTKALPRKSRDPLLSQRISSLTASLHAHSRKKVKRKHPRRAQDSKKPPRAPRTSKAQRRRLTGKARHSGE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 nucleolar protein 12
别名
计算分子量 25 kDa
观测分子量 32 kDa
GenBank蛋白编号BC002808
基因名称 NOL12
Gene ID (NCBI) 79159
RRIDAB_2282718
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UGY1
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Nucleolar protein 12 (NOL12), a multifunctional RNA-binding protein, has been found to be related to genomic stability, DNA damage repair, and apoptosis. High expression of NOL12 is associated with worse reduced overall survival (OS), high pathological grade, node metastasis, and advanced clinical stage in patients with HCC (PMID: 35693698). Knockdown of NOL12 impairs cellular proliferation by inducing a G1/S cell cycle arrest, but does so outside of a nucleolar stress response and in a p53-independent manner (PMID: 29069457).

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