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NOL12 Polyclonal antibody

NOL12 Polyclonal Antibody for IF/ICC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, IP, WB, ELISA and More (2)

Conjugate

Unconjugated

Cat No : 15456-1-AP

Print datasheet

Synonyms

NOL12, Nop25, nucleolar protein 12

验证数据展示

  • WB analysis using 15456-1-AP

    WB analysis using 15456-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15456-1-AP (NOL12 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 15456-1-AP

    WB analysis of HeLa using 15456-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 15456-1-AP (NOL12 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 15456-1-AP

    IP experiment of HeLa using 15456-1-AP

    IP result of anti-NOL12 (IP:15456-1-AP, 4ug; Detection:15456-1-AP 1:300) with HeLa cells lysate 4000ug.

  • IF Staining of HepG2 using 15456-1-AP

    IF Staining of HepG2 using 15456-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 15456-1-AP (NOL12 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHeLa cells, SH-SY5Y cells, HepG2 cells, Raji cells
Positive IP detected inHeLa cells
Positive IF detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15456-1-AP targets NOL12 in WB, IP, IF, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, IP, WB, ELISA
Cited ApplicationsWB, IF, IHC, CoIP
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, rat
Immunogen NOL12 fusion protein Ag7723 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name nucleolar protein 12
Synonyms NOL12, Nop25, nucleolar protein 12
Calculated Molecular Weight 25 kDa
Observed Molecular Weight 32 kDa
GenBank Accession NumberBC002808
Gene Symbol NOL12
Gene ID (NCBI) 79159
RRIDAB_2282718
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UGY1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for NOL12 antibody 15456-1-APDownload protocol
IF protocol for NOL12 antibody 15456-1-APDownload protocol
IP protocol for NOL12 antibody 15456-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nature

Global landscape of HIV-human protein complexes.

Authors - Stefanie Jäger
View Article
human,ratIHC,WB,IF,CoIP

Cell Death Discov

Protein expression of nucleolar protein 12 in the retina and its implication in protection of retina from UV irradiation damage

Authors - Jingtao Liu
  • KD Validated
View Article