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Host
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Species Reactivity
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Species Reactivity
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Conjugate
0
Conjugate
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Compatible Species
0
Conjugate
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验证数据展示

  • WB analysis using 68194-1-Ig (same clone as 68194-1-PBS)

    WB analysis using 68194-1-Ig (same clone as 68194-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68194-1-Ig (NNT antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68194-1-PBS in a different storage buffer formulation.

  • WB analysis using 68194-1-Ig (same clone as 68194-1-PBS)

    WB analysis using 68194-1-Ig (same clone as 68194-1-PBS)

    Various lysates (mouse heart tissue and mouse liver tissue were dissected from BALB/c mice) were subjected to SDS PAGE followed by western blot with 68194-1-Ig (NNT antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68194-1-PBS in a different storage buffer formulation.

  • IP experiment of HepG2 using 68194-1-Ig (same clone as 68194-1-PBS)

    IP experiment of HepG2 using 68194-1-Ig (same clone as 68194-1-PBS)

    IP result of anti-NNT (IP:68194-1-Ig, 4ug; Detection:68194-1-Ig 1:5000) with HepG2 cells lysate 960 ug. This data was developed using the same antibody clone with 68194-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver using 68194-1-Ig (same clone as 68194-1-PBS)

    IHC staining of human liver using 68194-1-Ig (same clone as 68194-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 68194-1-Ig (NNT antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68194-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NNT antibody included
  • IHC staining of human liver using 68194-1-Ig (same clone as 68194-1-PBS)

    IHC staining of human liver using 68194-1-Ig (same clone as 68194-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 68194-1-Ig (NNT antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68194-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NNT antibody included
  • IF Staining of HeLa using 68194-1-Ig (same clone as 68194-1-PBS)

    IF Staining of HeLa using 68194-1-Ig (same clone as 68194-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using NNT antibody (68194-1-Ig, Clone: 1F5H6 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68194-1-PBS in a different storage buffer formulation.

NNT Monoclonal antibody, PBS Only

NNT Monoclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 68194-1-PBS

产品说明书

CloneNo. 1F5H6

宿主/亚型

Mouse / IgG2b

种属反应性

Human, Mouse, Rat, Rabbit, Pig

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

NNT

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy NNT Ready-To-Use IHC Kit. NNT primary antibody included.

产品信息

68194-1-PBS targets NNT in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat, Rabbit, Pig samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 Human, Mouse, Rat, Rabbit, Pig
免疫原 NNT fusion protein Ag4259 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 nicotinamide nucleotide transhydrogenase
别名 NNT
计算分子量 1085 aa, 114 kDa
观测分子量114 kDa
GenBank蛋白编号BC032370
基因名称 NNT
Gene ID (NCBI) 23530
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ13423
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

NNT(nicotinamide nucleotide transhydrogenase) is a transmembrane protein and functions as a proton pumping transhydrogenase. The protein is present in both prokaryotes and eukaryotes and is located in the inner membrane of mitochondria. In prokaryotic cells, the enzyme is composed of α and β subunits of 54 and 48 kDa, respectively. In eukaryotic cells, the enzyme is usually composed of a single peptide of 114 kDa. Although NNT catalyzes the interconversion of NADH and NADPH, the forward reaction using the reducing power of NADH to regenerate NADPH would be favored under conditions of oxidative stress(PMID:16497723). It can exsit as a homodimer(PMID:21882037).