验证数据展示
产品信息
84876-5-PBS targets NNMT in WB, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human, rat samples.
| 经测试应用 | WB, FC (Intra), IP, Indirect ELISA Application Description |
| 经测试反应性 | human, rat |
| 免疫原 |
CatNo: Ag7197 Product name: Recombinant human NNMT protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-264 aa of BC000234 Sequence: MESGFTSKDTYLSHFNPRDYLEKYYKFGSRHSAESQILKHLLKNLFKIFCLDGVKGDLLIDIGSGPTIYQLLSACESFKEIVVTDYSDQNLQELEKWLKKEPEAFDWSPVVTYVCDLEGNRVKGPEKEEKLRQAVKQVLKCDVTQSQPLGAVPLPPADCVLSTLCLDAACPDLPTYCRALRNLGSLLKPGGFLVIMDALKSSYYMIGEQKFSSLPLGREAVEAAVKEAGYTIEWFEVISQSYSSTMANNEGLFSLVARKLSRPL 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | nicotinamide N-methyltransferase |
| 别名 | 242394B7, EC:2.1.1.1, Nicotinamide N-methyltransferase |
| 计算分子量 | 30 kDa |
| 观测分子量 | 30 kDa |
| GenBank蛋白编号 | BC000234 |
| 基因名称 | NNMT |
| Gene ID (NCBI) | 4837 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purfication |
| UNIPROT ID | P40261 |
| 储存缓冲液 | PBS only, pH 7.3. |
| 储存条件 | Store at -80°C. The product is shipped with ice packs. Upon receipt, store it immediately at -80°C |
背景介绍
NNMT can catalyze the N-methylation of nicotinamide using the universal methyl donor S-adenosyl-L-methionine to form N1-methylnicotinamide and S-adenosyl-L-homocysteine, a predominant nicotinamide/vitamin B3 clearance pathway (PMID: 21823666; 23455543; 8182091). It plays a central role in regulating cellular methylation potential, by consuming S-adenosyl-L-methionine and limiting its availability for other methyltransferases. Actively mediates genome-wide epigenetic and transcriptional changes through hypomethylation of repressive chromatin marks, such as H3K27me3 (PMID: 23455543; PMID: 26571212; PMID: 31043742).
