验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, K-562 cells, PC-3 cells, HepG2 cells |
| Positive IF/ICC detected in | HepG2 cells |
| Positive FC (Intra) detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
83986-2-RR targets NMI in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IF/ICC, FC (Intra), ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag34759 Product name: Recombinant human NMI protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-100 aa of BC001268 Sequence: MEADKDDTQQILKEHSPDEFIKDEQNKGLIDEITKKNIQLKKEIQKLETELQEATKEFQIKEDIPETKMKFLSVETPENDSQLSNISCSFQVSSKVPYEI 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | N-myc (and STAT) interactor |
| 别名 | 241135A9, N myc (and STAT) interactor, N myc and STAT interactor, N myc interactor, N-myc and STAT interactor |
| 计算分子量 | 35 kDa |
| 观测分子量 | 35 kDa |
| GenBank蛋白编号 | BC001268 |
| 基因名称 | NMI |
| Gene ID (NCBI) | 9111 |
| RRID | AB_3671560 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q13287 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
N-Myc and STAT Interactor protein (NMI) is an interferon inducible protein that interacts with NMYC and CMYC (members of the oncogene Myc family), and other transcription factors containing a Zip, HLH, or HLH-Zip motif. It is widely involved in the process of tumor growth, progression, and metastasis.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for NMI antibody 83986-2-RR | Download protocol |
| WB protocol for NMI antibody 83986-2-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
