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Host
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Species Reactivity
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Species Reactivity
0
Conjugate
0
Conjugate
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Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 85074-2-RR (same clone as 85074-2-PBS)

    WB analysis using 85074-2-RR (same clone as 85074-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85074-2-RR (NME1/2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85074-2-PBS in a different storage buffer formulation.

  • FC experiment of HEK-293T using 85074-2-RR (same clone as 85074-2-PBS)

    FC experiment of HEK-293T using 85074-2-RR (same clone as 85074-2-PBS)

    1x10^6 HEK-293T cells were intracellularly stained with 0.25 ug NME1/2 Recombinant antibody (85074-2-RR, Clone:242698C7) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 85074-2-PBS in a different storage buffer formulation.

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NME1/2 Recombinant monoclonal antibody, PBS Only

NME1/2 Uni-rAb® Recombinant Antibody for WB, FC (Intra), Indirect ELISA
Cat No. 85074-2-PBS

产品说明书

CloneNo. 242698C7

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, FC (Intra), Indirect ELISA

别名

NME2, 242698C7, C-myc purine-binding transcription factor PUF, EC:2.7.13.3, EC:2.7.4.6

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

85074-2-PBS targets NME1/2 in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, FC (Intra), Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag13913

Product name: Recombinant human NME2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-152 aa of BC002476

Sequence: MANLERTFIAIKPDGVQRGLVGEIIKRFEQKGFRLVAMKFLRASEEHLKQHYIDLKDRPFFPGLVKYMNSGPVVAMVWEGLNVVKTGRVMLGETNPADSKPGTIRGDFCIQVGRNIIHGSDSVKSAEKEISLWFKPEELVDYKSCAHDWVYE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 non-metastatic cells 2, protein (NM23B) expressed in
别名 NME2, 242698C7, C-myc purine-binding transcription factor PUF, EC:2.7.13.3, EC:2.7.4.6
计算分子量 152 aa, 17 kDa
观测分子量 17 kDa
GenBank蛋白编号BC002476
基因名称 NME2
Gene ID (NCBI) 4831
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP22392
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

NME2(non-metastatic cells 2, protein) is also named as NDKB(Nucleoside diphosphate kinase B), NM23B and belongs to the NDK family. It is involved in the phosphorylation of nucleoside diphosphates and interacts with membrane receptor and constituting a novel molecular regulatory mechanism of GPCR endocytosis. This protein has 2 isoforms produced by alternative splicing. Refer to epitope and protein detection, the antibody 85074-2-RR detects both NME1& NME2.