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NME2 Polyclonal antibody

NME2 Polyclonal Antibody for IF/ICC, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, IHC, WB,ELISA

Conjugate

Unconjugated

Cat No : 20493-1-AP

Print datasheet

Synonyms

NDK B, NDP kinase B, NDPK B, NDPKB, NME2, puf

验证数据展示

  • WB analysis of mouse kidney using 20493-1-AP

    WB analysis of mouse kidney using 20493-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 20493-1-AP (NME2 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse kidney using 20493-1-AP

    IHC staining of mouse kidney using 20493-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 20493-1-AP (NME2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HEK-293 using 20493-1-AP

    IF Staining of HEK-293 using 20493-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HEK-293 cells using 20493-1-AP (NME2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inmouse kidney tissue, Jurkat cells, mouse liver tissue
Positive IHC detected inmouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHEK-293 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 3 publications below

产品信息

The immunogen of 20493-1-AP is NME2 Fusion Protein expressed in E. coli.

Tested Applications IF/ICC, IHC, WB,ELISA
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Immunogen NME2 fusion protein Ag13913 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name non-metastatic cells 2, protein (NM23B) expressed in
Synonyms NDK B, NDP kinase B, NDPK B, NDPKB, NME2, puf
Calculated Molecular Weight 152 aa, 17 kDa
Observed Molecular Weight 17 kDa
GenBank Accession NumberBC002476
Gene Symbol NME2
Gene ID (NCBI) 4831
RRIDAB_10695634
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP22392
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NME2(non-metastatic cells 2, protein) is also named as NDKB(Nucleoside diphosphate kinase B), NM23B and belongs to the NDK family. It is involved in the phosphorylation of nucleoside diphosphates and interacts with membrane receptor and constituting a novel molecular regulatory mechanism of GPCR endocytosis. This protein has 2 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
WB protocol for NME2 antibody 20493-1-APDownload protocol
IHC protocol for NME2 antibody 20493-1-APDownload protocol
IF protocol for NME2 antibody 20493-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Hazard Mater

Integrative proteomics and metabolomics approach to elucidate metabolic dysfunction induced by silica nanoparticles in hepatocytes.

Authors - Ye Zhu
View Article
ratWB

Proteomics

FHL2-drived molecular network mediated Septin2 knockdown inducing apoptosis in mesangial cell.

Authors - Yang Lu
View Article
mouseWB

Biochem Biophys Res Commun

Nicotine induces Nme2-mediated apoptosis in mouse testes.

Authors - Yunqi Gu
View Article