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验证数据展示

  • FC experiment of human PBMCs using FITC-98340

    FC experiment of human PBMCs using FITC-98340

    1x10^6 FC receptor blocked human PBMCs were surface stained with CoraLite® Plus 647 Anti-Human NCAM1/CD56, and 5 ul FITC Plus Anti-Human NKp46/NCR1 Rabbit RecAb (FITC-98340, Clone: 242549D12) or FITC Plus Rabbit IgG Isotype Control RecAb (FITC-98136, Clone: 240953C9). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  • FC experiment of human PBMCs using FITC-98340

    FC experiment of human PBMCs using FITC-98340

    1x10^6 FC receptor blocked human PBMCs were surface stained with 5 ul FITC Plus Anti-Human NKp46/NCR1 Rabbit RecAb (FITC-98340, Clone: 242549D12) (red) or FITC Plus Rabbit IgG Isotype Control RecAb (FITC-98136, Clone: 240953C9) (blue). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

FITC Plus Anti-Human NKp46/NCR1 Rabbit Recombinant Antibody

NKp46/NCR1 Recombinant Antibody for FC
Cat No. FITC-98340

产品说明书

CloneNo. 242549D12

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

FC

别名

242549D12, CD335, hNKp46, LY94, Natural killer cell p46-related protein

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  FITC Plus
规格价格库存

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经过测试的应用

Positive FC detected inhuman PBMCs

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC)FC : 5 ul per 10^6 cells in a 100 µl suspension
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

产品信息

FITC-98340 targets NKp46/NCR1 in FC applications and shows reactivity with human samples.

经测试应用 FC Application Description
经测试反应性 human
免疫原 NKp46/NCR1 fusion protein Eg1364 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 natural cytotoxicity triggering receptor 1
别名 242549D12, CD335, hNKp46, LY94, Natural killer cell p46-related protein
计算分子量34kDa
基因名称 NCR1
Gene ID (NCBI) 9437
偶联类型 FITC Plus Fluorescent Dye
最大激发/发射波长495 nm / 524 nm
形式Liquid
纯化方式Protein A purification
UNIPROT IDO76036-1
储存缓冲液 PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
储存条件Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

NKp46, also known as NCR1 or CD335, is an Ig-like superfamily cell surface receptor that is highly conserved in mammals (PMID: 22021440). NKp46 is a type I transmembrane glycoprotein consisting of two extracellular Ig-like domains, a transmembrane domain, and an intracellular tail (PMID: 9730896). It is expressed on NK cells, rare T-cell subsets and a mucosal population of NKp46+ innate lymphoid cells (PMID: 9730896; 22021440). NKp46 is the major triggering receptor involved in the natural cytotoxicity (PMID: 10359120; 10092106; 15356098).

实验方案

Product Specific Protocols
FC protocol for FITC Plus NKp46/NCR1 antibody FITC-98340Download protocol
Standard Protocols
Click here to view our Standard Protocols