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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Conjugate
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验证数据展示

  • FC experiment of mouse splenocytes using CL405-98198

    FC experiment of mouse splenocytes using CL405-98198

    1x10^6 mouse splenocytes were surface stained with CoraLite® Plus 488 Anti-Mouse CD49b, and 0.25 ug CoraLite® Plus 405 Anti-Mouse NKp46/NCR1 Rabbit RecAb (CL405-98198, Clone: 241804A12) or CoraLite® Plus 405 Rabbit IgG Isotype Control RecAb (CL405-98136, Clone: 240953C9). Cells were not fixed.

  • FC experiment of mouse splenocytes using CL405-98198

    FC experiment of mouse splenocytes using CL405-98198

    1x10^6 mouse splenocytes were surface stained with 0.25 ug CoraLite® Plus 405 Anti-Mouse NKp46/NCR1 Rabbit RecAb (CL405-98198, Clone: 241804A12) (red) or CoraLite® Plus 405 Rabbit IgG Isotype Control RecAb (CL405-98136, Clone: 240953C9) (blue). Cells were not fixed.

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CoraLite® Plus 405 Anti-Mouse NKp46/NCR1 Rabbit Recombinant Antibody

NKp46/NCR1 Uni-rAb® Recombinant Antibody for FC
Cat No. CL405-98198

产品说明书

CloneNo. 241804A12

宿主/亚型

Rabbit / IgG

种属反应性

mouse

应用

FC

别名

Ncr1, 241804A12, Activating receptor 1, CD335, Ly94

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 405
规格价格库存

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经过测试的应用

Positive FC detected inmouse splenocytes
For other applications, we recommend the unconjugated version of this antibody, 98198-1-PBS

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC)FC : 0.25 ug per 10^6 cells in a 100 µl suspension
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL405-98198 targets NKp46/NCR1 in FC applications and shows reactivity with mouse samples.

经测试应用 FC Application Description
经测试反应性 mouse
免疫原

CatNo: Eg1825

Product name: Recombinant Mouse NKp46/NCR1 protein (rFc Tag)

Source: mammalian cells-derived, pHZ-KIsec-C-rFc

Tag: C-rFc

Domain: 17-255 aa of NM_010746.3

Sequence: QRINTEKETLPKPIIWAKPSIMVTNGNSVNIWCQGAQSASEYQLYFEGSFFALERPKPSRSMNKVRFFISQMTSHTAGIYTCFYQSGELWSKSSNPLKLVVTGLYDTPNLWVYPRPEVTLGENVTFFCQLKTATSKFFLLKERGSNHIQNKYGNIQAEFPMGPVTRAHRGTYRCFGSYNDYAWSFPSEPVTLLITGGVENSSLAPTDPTSSLDYWEFDLSTNESGLQKDSAFWDHTTQN

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 natural cytotoxicity triggering receptor 1
别名 Ncr1, 241804A12, Activating receptor 1, CD335, Ly94
计算分子量37kDa
GenBank蛋白编号NM_010746.3
基因名称 NKp46/NCR1
Gene ID (NCBI) 17086
偶联类型 CoraLite® Plus 405 Fluorescent Dye
最大激发/发射波长399 nm / 422 nm
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ8C567
储存缓冲液 PBS with 0.09% sodium azide, pH 7.3.
储存条件Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

NKp46, also known as NCR1 or CD335, is an Ig-like superfamily cell surface receptor that is highly conserved in mammals (PMID: 22021440). NKp46 is a type I transmembrane glycoprotein consisting of two extracellular Ig-like domains, a transmembrane domain, and an intracellular tail (PMID: 9730896). It is expressed on NK cells, rare T-cell subsets and a mucosal population of NKp46+ innate lymphoid cells (PMID: 9730896; 22021440). NKp46 is the major triggering receptor involved in the natural cytotoxicity (PMID: 10359120; 10092106; 15356098).

实验方案

Product Specific Protocols
FC protocol for CL Plus 405 NKp46/NCR1 antibody CL405-98198Download protocol
Standard Protocols
Click here to view our Standard Protocols