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验证数据展示

  • WB analysis of mouse splenocytes using 84416-5-RR

    WB analysis of mouse splenocytes using 84416-5-RR

    mouse splenocytes were subjected to SDS PAGE followed by western blot with 84416-5-RR (NKp46/NCR1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IF Staining of rat spleen using 84416-5-RR

    IF Staining of rat spleen using 84416-5-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat spleen tissue using NKp46/NCR1 antibody (84416-5-RR, Clone: 241811H7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of rat spleen using 84416-5-RR

    IF Staining of rat spleen using 84416-5-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat spleen tissue using NKp46/NCR1 antibody (84416-5-RR, Clone: 241811H7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat spleen using 84416-5-RR

    IHC staining of rat spleen using 84416-5-RR

    Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 84416-5-RR (NKp46/NCR1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat spleen using 84416-5-RR

    IHC staining of rat spleen using 84416-5-RR

    Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 84416-5-RR (NKp46/NCR1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat thymus using 84416-5-RR

    IHC staining of rat thymus using 84416-5-RR

    Immunohistochemical analysis of paraffin-embedded rat thymus tissue slide using 84416-5-RR (NKp46/NCR1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 84416-5-RR

    Affinity and Kinetic Characterization of 84416-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84416-5-RR against Rat NKp46/NCR1 were performed. The affinity constant is 0.593 nM.

NKp46/NCR1 Recombinant monoclonal antibody

NKp46/NCR1 Uni-rAb® Recombinant Antibody for WB, IHC, IF-P, ELISA
Cat No. 84416-5-RR

产品说明书

CloneNo. 241811H7

宿主/亚型

Rabbit / IgG

种属反应性

mouse, rat

应用

WB, IHC, IF-P, ELISA

别名

241811H7, Activating receptor 1, Ar1, CD335, Kilr1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse splenocytes
Positive IHC detected inrat spleen tissue, rat thymus tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inrat spleen tissue, mouse spleen tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84416-5-RR targets NKp46/NCR1 in WB, IHC, IF-P, ELISA applications and shows reactivity with mouse, rat samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
经测试反应性 mouse, rat
免疫原

CatNo: Eg1469

Product name: Recombinant Rat NKp46/NCR1 protein (rFc Tag)

Source: mammalian cells-derived, pHZ-KIsec-C-rFc

Tag: C-rFc

Domain: 17-258 aa of NM_057199.1

Sequence: QRINTEKQTLPKPIIWAKPSIMVTKGNSVNIWCQGAQSASEYQLYFEGSFFALERPKSSRSMNKVKFFISQMTSHTAGIYTCFYQSGELWSESSNPLKLVVTGLYDTPTLWVHPGPEVTLGENVTFSCHLKTATSKFFLLKERESNHIQHKYGNIQAEFPMGPVTRAHRGTYRCFGSYNDYAWSFPSEPVTLLITGEVENTSLAPTDPVSSLDYWEFDLSTKESGLQKDSAFWDHTAQNLIR

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 natural cytotoxicity triggering receptor 1
别名 241811H7, Activating receptor 1, Ar1, CD335, Kilr1
计算分子量37kDa
观测分子量50-60 kDa
GenBank蛋白编号NM_057199.1
基因名称 NKp46/NCR1
Gene ID (NCBI) 117547
RRIDAB_3671945
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDQ9Z0H5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NKp46, also known as NCR1 or CD335, is an Ig-like superfamily cell surface receptor that is highly conserved in mammals (PMID: 22021440). NKp46 is a type I transmembrane glycoprotein consisting of two extracellular Ig-like domains, a transmembrane domain, and an intracellular tail (PMID: 9730896). It is expressed on NK cells, rare T-cell subsets, and a mucosal population of NKp46+ innate lymphoid cells (PMID: 9730896; 22021440). NKp46 is the major triggering receptor involved in the natural cytotoxicity (PMID: 10359120; 10092106; 15356098).

实验方案

Product Specific Protocols
IF protocol for NKp46/NCR1 antibody 84416-5-RRDownload protocol
IHC protocol for NKp46/NCR1 antibody 84416-5-RRDownload protocol
WB protocol for NKp46/NCR1 antibody 84416-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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