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验证数据展示

  • WB analysis using 68230-1-Ig (same clone as 68230-1-PBS)

    WB analysis using 68230-1-Ig (same clone as 68230-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68230-1-Ig (NKRF antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 68230-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 68230-1-Ig (same clone as 68230-1-PBS)

    WB analysis of HEK-293 using 68230-1-Ig (same clone as 68230-1-PBS)

    WB result of NKRF antibody (68230-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NKRF transfected HEK-293 cells. This data was developed using the same antibody clone with 68230-1-PBS in a different storage buffer formulation.

  • WB analysis using 68230-1-Ig (same clone as 68230-1-PBS)

    WB analysis using 68230-1-Ig (same clone as 68230-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68230-1-Ig (NKRF antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 68230-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

NKRF Monoclonal antibody, PBS Only

NKRF Monoclonal Antibody for WB, Indirect ELISA
Cat No. 68230-1-PBS

产品说明书

CloneNo. 1H10C8

宿主/亚型

Mouse / IgG1

种属反应性

Human, Rat, Mouse

应用

WB, Indirect ELISA

别名

ITBA4, NF kappa B repressing factor, NFKB repressing factor, NKRF, NRF, Protein ITBA4, Transcription factor NRF

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

68230-1-PBS targets NKRF in WB, Indirect ELISA applications and shows reactivity with Human, Rat, Mouse samples.

经测试应用 WB, Indirect ELISA Application Description
经测试反应性 Human, Rat, Mouse
免疫原

CatNo: Ag6374

Product name: Recombinant human NKRF protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 345-690 aa of BC068514

Sequence: NANDAIGILNNSASFNKMSIEYKYEMMPNRTWRCRVFLQDHCLAEGYGTKKTSKHAAADEALKILQKTQPTYPSVKSSQCHTGSSPRGSGKKKDIKDLVVYENSSNPVCTLNDTAQFNRMTVEYVYERMTGLRWKCKVILESEVIAEAVGVKKTVKYEAAGEAVKTLKKTQPTVINNLKKGAVEDVISRNEIQGRSAEEAYKQQIKEDNIGNQLLRKMGWTGGGLGKSGEGIREPISVKEQHKREGLGLDVERVNKIAKRDIEQIIRNYARSESHTDLTFSRELTNDERKQIHQIAQKYGLKSKSHGVGHDRYLVVGRKRRKEDLLDQLKQEGQVGHYELVMPQAN

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 NFKB repressing factor
别名 ITBA4, NF kappa B repressing factor, NFKB repressing factor, NKRF, NRF, Protein ITBA4, Transcription factor NRF
计算分子量 78 kDa
观测分子量100 kDa
GenBank蛋白编号BC068514
基因名称 NKRF
Gene ID (NCBI) 55922
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDO15226
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

NKRF, also named as Protein ITBA4 or Transcription factor NRF, is a 690 amino acid protein, which contains one G-patch domain and one R3H domain. NKRF is Widely and constitutively expressed, with lower level in colon, peripheral blood lymphocytes, lung and kidney. NKRF interacts with a specific negative regulatory element (NRE) 5'-AATTCCTCTGA-3' to mediate transcriptional repression of certain NK-kappa-B responsive genes. NKRF is involved in the constitutive silencing of the IFN-β promoter, independently of the virus-induced signals, and in the inhibition of the basal and cytokine-induced iNOS promoter activity.