• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
Use Able AI chat for product recommendations

验证数据展示

  • WB analysis using 86201-1-RR

    WB analysis using 86201-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86201-1-RR (NKCC1/SLC12A2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 86201-1-RR

    IHC staining of human colon cancer using 86201-1-RR

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 86201-1-RR (NKCC1/SLC12A2 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon cancer using 86201-1-RR

    IHC staining of human colon cancer using 86201-1-RR

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 86201-1-RR (NKCC1/SLC12A2 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon using 86201-1-RR

    IHC staining of human colon using 86201-1-RR

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 86201-1-RR (NKCC1/SLC12A2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon using 86201-1-RR

    IHC staining of human colon using 86201-1-RR

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 86201-1-RR (NKCC1/SLC12A2 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 86201-1-RR

    Affinity and Kinetic Characterization of 86201-1-RR

    Biolayer interferometry (BLl) kinetic assays of 86201-1-RR against Human NKCC1/SLC12A2 were performed. The affinity constant is below 1 pM.

NKCC1/SLC12A2 Recombinant monoclonal antibody

NKCC1/SLC12A2 Uni-rAb® Recombinant Antibody for WB, IHC, ELISA
Cat No. 86201-1-RR

产品说明书

CloneNo. 250829D8

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

NKCC1, SLC12A2, Basolateral Na K Cl symporter, Basolateral Na-K-Cl symporter, BSC

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected in37°C incubated mouse brain tissue
Positive IHC detected inhuman colon cancer tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

86201-1-RR targets NKCC1/SLC12A2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag4991

Product name: Recombinant human SLC12A2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 759-1150 aa of BC033003

Sequence: GSSTQALTYLNALQHSIRLSGVEDHVKNFRPQCLVMTGAPNSRPALLHLVHDFTKNVGLMICGHVHMGPRRQAMKEMSIDQAKYQRWLIKNKMKAFYAPVHADDLREGAQYLMQAAGLGRMKPNTLVLGFKKDWLQADMRDVDMYINLFHDAFDIQYGVVVIRLKEGLDISHLQGQEELLSSQEKSPGTKDVVVSVEYSKKSDLDTSKPLSEKPITHKVEEEDGKTATQPLLKKESKGPIVPLNVADQKLLEASTQFQKKQGKNTIDVWWLFDDGGLTLLIPYLLTTKKKWKDCKIRVFIGGKINRIDHDRRAMATLLSKFRIDFSDIMVLGDINTKPKKENIIAFEEIIEPYRLHEDDKEQDIADKMKEDEPWRITDNELELYKTKFYEPC

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 solute carrier family 12 (sodium/potassium/chloride transporters), member 2
别名 NKCC1, SLC12A2, Basolateral Na K Cl symporter, Basolateral Na-K-Cl symporter, BSC
计算分子量 131 kDa
观测分子量 150-160 kDa
GenBank蛋白编号BC033003
基因名称 NKCC1/SLC12A2
Gene ID (NCBI) 6558
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP55011
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The Na-K-Cl cotransporter sodium-potassium-chloride cotransporter 1 (NKCC1), encoded by the SLC12A2 gene, is an intensively studied member of the CCC family. NKCC1 is a membrane protein and plays fundamental roles in regulating trans-epithelial ion movement, cell volume, chloride homeostasis and neuronal excitability. NKCC1 assembles into a dimer, with the first ten transmembrane (TM) helices harboring the transport core and TM11-TM12 helices lining the dimer interface(PMID: 32081947). The observed molecular weight of NKCC1 is about 140 kDa, glycosylated NKCC1 is detected a band at ∼170 kDa (PMID: 22723696).

实验方案

Product Specific Protocols
IHC protocol for NKCC1/SLC12A2 antibody 86201-1-RRDownload protocol
WB protocol for NKCC1/SLC12A2 antibody 86201-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
||
New chat Able
正在加载，请稍候...