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验证数据展示

  • WB analysis using 86201-1-RR (same clone as 86201-1-PBS)

    WB analysis using 86201-1-RR (same clone as 86201-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86201-1-RR (NKCC1/SLC12A2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86201-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 86201-1-RR (same clone as 86201-1-PBS)

    IHC staining of human colon cancer using 86201-1-RR (same clone as 86201-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 86201-1-RR (NKCC1/SLC12A2 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86201-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 86201-1-RR (same clone as 86201-1-PBS)

    IHC staining of human colon cancer using 86201-1-RR (same clone as 86201-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 86201-1-RR (NKCC1/SLC12A2 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86201-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon using 86201-1-RR (same clone as 86201-1-PBS)

    IHC staining of human colon using 86201-1-RR (same clone as 86201-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 86201-1-RR (NKCC1/SLC12A2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86201-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon using 86201-1-RR (same clone as 86201-1-PBS)

    IHC staining of human colon using 86201-1-RR (same clone as 86201-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 86201-1-RR (NKCC1/SLC12A2 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86201-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 86201-1-RR

    Affinity and Kinetic Characterization of 86201-1-RR

    Biolayer interferometry (BLl) kinetic assays of 86201-1-RR against Human NKCC1/SLC12A2 were performed. The affinity constant is below 1 pM.

NKCC1/SLC12A2 Recombinant monoclonal antibody, PBS Only

NKCC1/SLC12A2 Uni-rAb® Recombinant Antibody for WB, IHC, Indirect ELISA
Cat No. 86201-1-PBS

产品说明书

CloneNo. 250829D8

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, Indirect ELISA

别名

NKCC1, SLC12A2, Basolateral Na K Cl symporter, Basolateral Na-K-Cl symporter, BSC

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

86201-1-PBS targets NKCC1/SLC12A2 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag4991

Product name: Recombinant human SLC12A2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 759-1150 aa of BC033003

Sequence: GSSTQALTYLNALQHSIRLSGVEDHVKNFRPQCLVMTGAPNSRPALLHLVHDFTKNVGLMICGHVHMGPRRQAMKEMSIDQAKYQRWLIKNKMKAFYAPVHADDLREGAQYLMQAAGLGRMKPNTLVLGFKKDWLQADMRDVDMYINLFHDAFDIQYGVVVIRLKEGLDISHLQGQEELLSSQEKSPGTKDVVVSVEYSKKSDLDTSKPLSEKPITHKVEEEDGKTATQPLLKKESKGPIVPLNVADQKLLEASTQFQKKQGKNTIDVWWLFDDGGLTLLIPYLLTTKKKWKDCKIRVFIGGKINRIDHDRRAMATLLSKFRIDFSDIMVLGDINTKPKKENIIAFEEIIEPYRLHEDDKEQDIADKMKEDEPWRITDNELELYKTKFYEPC

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 solute carrier family 12 (sodium/potassium/chloride transporters), member 2
别名 NKCC1, SLC12A2, Basolateral Na K Cl symporter, Basolateral Na-K-Cl symporter, BSC
计算分子量 131 kDa
观测分子量 150-160 kDa
GenBank蛋白编号BC033003
基因名称 NKCC1/SLC12A2
Gene ID (NCBI) 6558
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP55011
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The Na-K-Cl cotransporter sodium-potassium-chloride cotransporter 1 (NKCC1), encoded by the SLC12A2 gene, is an intensively studied member of the CCC family. NKCC1 is a membrane protein and plays fundamental roles in regulating trans-epithelial ion movement, cell volume, chloride homeostasis and neuronal excitability. NKCC1 assembles into a dimer, with the first ten transmembrane (TM) helices harboring the transport core and TM11-TM12 helices lining the dimer interface(PMID: 32081947). The observed molecular weight of NKCC1 is about 140 kDa, glycosylated NKCC1 is detected a band at ∼170 kDa (PMID: 22723696).

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