验证数据展示
经过测试的应用
| Positive WB detected in | SH-SY5Y cells, Caco-2 cells, HeLa cells |
| Positive IP detected in | HepG2 cells |
| Positive IHC detected in | human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
15128-1-AP targets NHP2 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP, CoIP |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse, yeast |
| 免疫原 |
CatNo: Ag7251 Product name: Recombinant human NHP2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-153 aa of BC000009 Sequence: MTKIKADPDGPEAQAEACSGERTYQELLVNQNPIAQPLASRRLTRKLYKCIKKAVKQKQIRRGVKEVQKFVNKGEKGIMVLAGDTLPIEVYCHLPVMCEDRNLPYVYIPSKTDLGAAAGSKRPTCVIMVKPHEEYQEAYDECLEEVQSLPLPL 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | NHP2 ribonucleoprotein homolog (yeast) |
| 别名 | HSPC286, NHP 2, NHP2P, NOLA2 |
| 计算分子量 | 17 kDa |
| 观测分子量 | 17-20 kDa |
| GenBank蛋白编号 | BC000009 |
| 基因名称 | NHP2 |
| Gene ID (NCBI) | 55651 |
| RRID | AB_2267290 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9NX24 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
NHP2 belongs to the H/ACA small nucleolar ribonucleoprotein (H/ACA snoRNP) complex, which catalyzes pseudouridylation of rRNA. It is required for ribosome biogenesis and telomere maintenance, and for correct processing or intranuclear trafficking of TERC, the RNA component of the telomerase reverse transcriptase (TERT) holoenzyme.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for NHP2 antibody 15128-1-AP | Download protocol |
| IHC protocol for NHP2 antibody 15128-1-AP | Download protocol |
| IP protocol for NHP2 antibody 15128-1-AP | Download protocol |
| WB protocol for NHP2 antibody 15128-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Science Cryo-EM structure of human telomerase dimer reveals H/ACA RNP-mediated dimerization | ||
Cell snoRNA-facilitated protein secretion revealed by transcriptome-wide snoRNA target identification | ||
Nucleic Acids Res N-terminal residues of human dyskerin are required for interactions with telomerase RNA that prevent RNA degradation. |
