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验证数据展示

  • WB analysis of mouse cerebellum using 25598-1-AP (same clone as 25598-1-PBS)

    WB analysis of mouse cerebellum using 25598-1-AP (same clone as 25598-1-PBS)

    mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 25598-1-AP (NFU1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25598-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse brain using 25598-1-AP (same clone as 25598-1-PBS)

    IP experiment of mouse brain using 25598-1-AP (same clone as 25598-1-PBS)

    IP result of anti-NFU1 (IP:25598-1-AP, 4ug; Detection:25598-1-AP 1:1000) with mouse brain tissue lysate 4000ug. This data was developed using the same antibody clone with 25598-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 25598-1-AP (same clone as 25598-1-PBS)

    IF Staining of HepG2 using 25598-1-AP (same clone as 25598-1-PBS)

    Immunofluorescent analysis of HepG2 cells using 25598-1-AP (NFU1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 25598-1-PBS in a different storage buffer formulation.

NFU1 Polyclonal antibody, PBS Only

NFU1 Polyclonal Antibody for WB, IF/ICC, IP, Indirect ELISA
Cat No. 25598-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, IP, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

25598-1-PBS targets NFU1 in WB, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag22343

Product name: Recombinant human NFU1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-254 aa of BC113692

Sequence: MAATARRGWGAAAVAAGLRRRFCHMLKNPYTIKKQPLHQFVQRPLFPLPAAFYHPVRYMFIQTQDTPNPNSLKFIPGKPVLETRTMDFPTPAAAFRSPLARQLFRIEGVKSVFFGPDFITVTKENEELDWNLLKPDIYATIMDFFASGLPLVTEETPSGEAGSEEDDEVVAMIKELLDTRIRPTVQEDGGDVIYKGFEDGIVQLKLQGSCTSCPSSIITLKNGIQNMLQFYIPEVEGVEQVMDDESDEKEANSP

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 NFU1 iron-sulfur cluster scaffold homolog (S. cerevisiae)
别名
计算分子量 254 aa, 28 kDa
观测分子量 23-28 kDa
GenBank蛋白编号BC113692
基因名称 NFU1
Gene ID (NCBI) 27247
RRIDAB_2880151
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UMS0
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

NFU1, also named as HIRA-interacting protein 5 or CGI-33, is a 254 amino acid protein, which belongs to the NifU family. NFU1 localizes in the Mitochondrion. NFU1 is widely expressed in adult tissues. NFU1, as an Iron-sulfur cluster scaffold protein, which can assemble clusters and deliver them to target proteins. It has an association with Multiple mitochondrial dysfunctions syndrome 1.

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