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NFU1 Polyclonal antibody

NFU1 Polyclonal Antibody for IF/ICC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, IP, WB,ELISA

Conjugate

Unconjugated

Cat No : 25598-1-AP

Print datasheet

Synonyms

CGI 33, HIRA interacting protein 5, HIRIP5, Nfu, NFU1, NifU, NIFUC

验证数据展示

  • WB analysis of mouse cerebellum using 25598-1-AP

    WB analysis of mouse cerebellum using 25598-1-AP

    mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 25598-1-AP (NFU1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse brain using 25598-1-AP

    IP experiment of mouse brain using 25598-1-AP

    IP result of anti-NFU1 (IP:25598-1-AP, 4ug; Detection:25598-1-AP 1:1000) with mouse brain tissue lysate 4000ug.

  • IF Staining of HepG2 using 25598-1-AP

    IF Staining of HepG2 using 25598-1-AP

    Immunofluorescent analysis of HepG2 cells using 25598-1-AP (NFU1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inmouse cerebellum tissue, mouse brain tissue, mouse testis tissue
Positive IP detected inmouse brain tissue
Positive IF detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

The immunogen of 25598-1-AP is NFU1 Fusion Protein expressed in E. coli.

Tested Applications IF/ICC, IP, WB,ELISA
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen NFU1 fusion protein Ag22343 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name NFU1 iron-sulfur cluster scaffold homolog (S. cerevisiae)
Synonyms CGI 33, HIRA interacting protein 5, HIRIP5, Nfu, NFU1, NifU, NIFUC
Calculated Molecular Weight 254 aa, 28 kDa
Observed Molecular Weight 23-28 kDa
GenBank Accession NumberBC113692
Gene Symbol NFU1
Gene ID (NCBI) 27247
RRIDAB_2880151
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UMS0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NFU1, also named as HIRA-interacting protein 5 or CGI-33, is a 254 amino acid protein, which belongs to the NifU family. NFU1 localizes in the Mitochondrion. NFU1 is widely expressed in adult tissues. NFU1, as an Iron-sulfur cluster scaffold protein, which can assemble clusters and deliver them to target proteins. It has an association with Multiple mitochondrial dysfunctions syndrome 1.

实验方案

Product Specific Protocols
WB protocol for NFU1 antibody 25598-1-APDownload protocol
IF protocol for NFU1 antibody 25598-1-APDownload protocol
IP protocol for NFU1 antibody 25598-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Science

Autoregulatory control of mitochondrial glutathione homeostasis

Authors - Yuyang Liu
View Article