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- KD/KO Validated
CoraLite® Plus 488-conjugated NFS1 Monoclonal antibody
NFS1 Monoclonal Antibody for FC (Intra), IF/ICC
Host / Isotype
Mouse / IgG1
Reactivity
Human, Mouse, Rat
Applications
FC (Intra), IF/ICC
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
3A10A7
验证数据展示
经过测试的应用
| Positive IF detected in | HeLa cells |
| Positive FC detected in | HepG2 cells |
For other applications, we recommend the unconjugated version of this antibody, 67021-1-Ig
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-67021 targets NFS1 in IF, FC (Intra) applications and shows reactivity with Human, Mouse, Rat samples.
| Tested Applications | FC (Intra), IF/ICC |
| Tested Reactivity | Human, Mouse, Rat |
| Immunogen | NFS1 fusion protein Ag8017 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | NFS1 nitrogen fixation 1 homolog (S. cerevisiae) |
| Synonyms | HUSSY 08, IscS, NFS1, NIFS |
| Calculated Molecular Weight | 50 kDa |
| GenBank Accession Number | BC018471 |
| Gene Symbol | NFS1 |
| Gene ID (NCBI) | 9054 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q9Y697 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
NFS1(nitrogen fixation 1 homolog) is also named as NIFS, HUSSY-08 and belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. The protein has been identified as a pyridoxal phosphate-containing homodimer that catalyzes the formation of equimolar amounts of elemental sulfur and L-alanine from the substrate, L-cysteine. It is reported that NFS1 is also able to catalyze the removal of selenium from selenocysteine, a mechanism similar to the L-cysteine reaction was postulated(PMID:9812986). It has 2 isoforms produced by alternative initiation.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 NFS1 antibody CL488-67021 | Download protocol |
| FC protocol for CL Plus 488 NFS1 antibody CL488-67021 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
