• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis of human skeletal muscle using 66368-1-Ig (same clone as 66368-1-PBS)

    WB analysis of human skeletal muscle using 66368-1-Ig (same clone as 66368-1-PBS)

    Western blot analysis of NFIL3 in various cell lines and tissues using Proteintech antibody 66368-1-Ig at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66368-1-PBS in a different storage buffer formulation.

  • FC experiment of NK92 using 66368-1-Ig (same clone as 66368-1-PBS)

    FC experiment of NK92 using 66368-1-Ig (same clone as 66368-1-PBS)

    1X10^6 NK92 were intracellularly stained with 0.4 ug Anti-Human NFIL3 (66368-1-Ig, Clone:2F4B4) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug x. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 66368-1-PBS in a different storage buffer formulation.

NFIL3 Monoclonal antibody, PBS Only

NFIL3 Monoclonal Antibody for ELISA, WB
Cat No. 66368-1-PBS

产品说明书

CloneNo. 2F4B4

宿主/亚型

Mouse / IgG1

种属反应性

pig, human

应用

ELISA, WB

别名

Interleukin-3-binding protein 1, Interleukin-3 promoter transcriptional activator, IL3BP1, E4BP4, E4 promoter-binding protein 4

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

66368-1-Ig

产品信息

66368-1-PBS targets NFIL3 in ELISA, WB applications and shows reactivity with pig, human samples.

经测试应用 ELISA, WB Application Description
经测试反应性 pig, human
免疫原 NFIL3 fusion protein Ag2380 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 nuclear factor, interleukin 3 regulated
别名 Interleukin-3-binding protein 1, Interleukin-3 promoter transcriptional activator, IL3BP1, E4BP4, E4 promoter-binding protein 4
计算分子量 462 aa, 51 kDa
观测分子量 50-55 kDa
GenBank蛋白编号BC008197
基因名称 NFIL3
Gene ID (NCBI) 4783
RRIDAB_2881748
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ16649
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

NFIL3, also named E4BP4 and IL3BP1, belongs to the bZIP family and NFIL3 subfamily. It represses transcription from promoters with activating transcription factor (ATF) sites. NFIL3 activates transcription from the interleukin-3 promoter in T-cells. It competes for the same consensus-binding site with PAR DNA-binding factors (DBP, HLF and TEF). It is a component of the circadian clock that acts as a negative regulator for the circadian expression of PER2 oscillation in the cell-autonomous core clock. NFIL3 protects pro-B cells from programmed cell death. It is a key regulator of TH2 responses(PMID:21499227).