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验证数据展示

  • WB analysis of human skeletal muscle using 66368-1-Ig

    WB analysis of human skeletal muscle using 66368-1-Ig

    Western blot analysis of NFIL3 in various cell lines and tissues using Proteintech antibody 66368-1-Ig at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • FC experiment of NK92 using 66368-1-Ig

    FC experiment of NK92 using 66368-1-Ig

    1X10^6 NK92 were intracellularly stained with 0.4 ug Anti-Human NFIL3 (66368-1-Ig, Clone:2F4B4) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug x. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

NFIL3 Monoclonal antibody

NFIL3 Monoclonal Antibody for WB, FC (Intra), ELISA
Cat No. 66368-1-Ig

产品说明书

CloneNo. 2F4B4

宿主/亚型

Mouse / IgG1

种属反应性

human, pig

应用

WB, FC (Intra), ELISA

别名

Interleukin-3-binding protein 1, Interleukin-3 promoter transcriptional activator, IL3BP1, E4BP4, E4 promoter-binding protein 4

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman skeletal muscle tissue
Positive FC (Intra) detected inNK92, NK92 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66368-1-Ig targets NFIL3 in WB, FC (Intra), ELISA applications and shows reactivity with human, pig samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human, pig
免疫原 NFIL3 fusion protein Ag2380 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 nuclear factor, interleukin 3 regulated
别名 Interleukin-3-binding protein 1, Interleukin-3 promoter transcriptional activator, IL3BP1, E4BP4, E4 promoter-binding protein 4
计算分子量 462 aa, 51 kDa
观测分子量 50-55 kDa
GenBank蛋白编号BC008197
基因名称 NFIL3
Gene ID (NCBI) 4783
RRIDAB_2881748
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ16649
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NFIL3, also named E4BP4 and IL3BP1, belongs to the bZIP family and NFIL3 subfamily. It represses transcription from promoters with activating transcription factor (ATF) sites. NFIL3 activates transcription from the interleukin-3 promoter in T-cells. It competes for the same consensus-binding site with PAR DNA-binding factors (DBP, HLF and TEF). It is a component of the circadian clock that acts as a negative regulator for the circadian expression of PER2 oscillation in the cell-autonomous core clock. NFIL3 protects pro-B cells from programmed cell death. It is a key regulator of TH2 responses(PMID:21499227).

实验方案

Product Specific Protocols
WB protocol for NFIL3 antibody 66368-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols