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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 68318-1-Ig (same clone as 68318-1-PBS)

    WB analysis using 68318-1-Ig (same clone as 68318-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68318-1-Ig (NFIC antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68318-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 68318-1-Ig (same clone as 68318-1-PBS)

    FC experiment of HeLa using 68318-1-Ig (same clone as 68318-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug NFIC Monoclonal antibody (68318-1-Ig, Clone:1A11A5) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.25 ug Mouse IgG2b isotype control Mouse McAb (66360-3-Ig, Clone: 11B8C4) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 68318-1-PBS in a different storage buffer formulation.

NFIC Monoclonal antibody, PBS Only

NFIC Monoclonal Antibody for WB, FC (Intra), Indirect ELISA
Cat No. 68318-1-PBS

产品说明书

CloneNo. 1A11A5

宿主/亚型

Mouse / IgG2b

种属反应性

human, mouse, rat

应用

WB, FC (Intra), Indirect ELISA

别名

NF-I/C, NF1-C, NF I, CTF, CCAAT-box-binding transcription factor

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy NFIC Ready-To-Use IHC Kit. NFIC primary antibody included.

产品信息

68318-1-PBS targets NFIC in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, FC (Intra), Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 NFIC fusion protein Ag9472 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 nuclear factor I/C (CCAAT-binding transcription factor)
别名 NF-I/C, NF1-C, NF I, CTF, CCAAT-box-binding transcription factor
计算分子量428aa,48 kDa; 508aa,57 kDa
观测分子量 50-56 kDa
GenBank蛋白编号BC012120
基因名称 NFIC
Gene ID (NCBI) 4782
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP08651
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

NFIC, also named as Nuclear factor 1 C-type, is a 508 amino acid protein, which contains 1 CTF/NF-I DNA-binding domain and belongs to the CTF/NF-I family. NFIC localizes in the nucleus and recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication.