• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

NFIC Polyclonal antibody

NFIC Polyclonal Antibody for IF/ICC, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, IHC, IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 16399-1-AP

Print datasheet

Synonyms

CTF, CTF5, NF I, NF I/C, NF1 C, NFI, NFI C, NFIC, Nuclear factor 1 C type, Nuclear factor 1/C, Nuclear factor I/C, TGGCA binding protein

验证数据展示

  • IF Staining of PC-3 using 16399-1-AP

    IF Staining of PC-3 using 16399-1-AP

    Immunofluorescent analysis of (4% PFA) fixed PC-3 cells using 16399-1-AP (NFIC antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • WB analysis of PC-3 using 16399-1-AP

    WB analysis of PC-3 using 16399-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 16399-1-AP (NFIC Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 16399-1-AP

    IP experiment of HeLa using 16399-1-AP

    IP result of anti-NFIC (IP:16399-1-AP, 4ug; Detection:16399-1-AP 1:1000) with HeLa cells lysate 3600ug.

  • IHC staining of human liver cancer using 16399-1-AP

    IHC staining of human liver cancer using 16399-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16399-1-AP (NFIC antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with NFIC antibody included
  • IHC staining of human liver cancer using 16399-1-AP

    IHC staining of human liver cancer using 16399-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16399-1-AP (NFIC antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with NFIC antibody included

经过测试的应用

Positive WB detected inPC-3 cells, mouse brain tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inPC-3 cells
Planning an IHC experiment? We recommend our IHCeasy NFIC Ready-To-Use IHC Kit. NFIC primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16399-1-AP targets NFIC in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, IHC, IP, WB, ELISA
Cited ApplicationsWB, IP, IHC
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen NFIC fusion protein Ag9494 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name nuclear factor I/C (CCAAT-binding transcription factor)
Synonyms CTF, CTF5, NF I, NF I/C, NF1 C, NFI, NFI C, NFIC, Nuclear factor 1 C type, Nuclear factor 1/C, Nuclear factor I/C, TGGCA binding protein
Calculated Molecular Weight428aa,48 kDa; 508aa,57 kDa
Observed Molecular Weight 56 kDa
GenBank Accession NumberBC012120
Gene Symbol NFIC
Gene ID (NCBI) 4782
RRIDAB_2878252
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP08651
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NFIC, also named as Nuclear factor 1 C-type, is a 508 amino acid protein, which contains 1 CTF/NF-I DNA-binding domain and belongs to the CTF/NF-I family. NFIC localizes in the nucleus and recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication.

实验方案

Product Specific Protocols
WB protocol for NFIC antibody 16399-1-APDownload protocol
IHC protocol for NFIC antibody 16399-1-APDownload protocol
IF protocol for NFIC antibody 16399-1-APDownload protocol
IP protocol for NFIC antibody 16399-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIHC

J Dent Res

Essential Role of IFT140 in Promoting Dentinogenesis.

Authors - G Li
View Article
humanWB,IHC

J Bone Miner Res

METTL3-mediated m6 A mRNA methylation modulates tooth root formation via affecting NFIC translation.

Authors - Rui Sheng
View Article
human,mouseWB,IHC,IP

Int J Oral Sci

USP34 regulates tooth root morphogenesis by stabilizing NFIC.

Authors - Shuang Jiang
View Article
humanWB

Mol Carcinog

Neuroplastin-β mediates S100A8/A9-induced lung cancer disseminative progression.

Authors - I Wayan Sumardika
View Article