×
Use Able AI chat for product recommendations

验证数据展示

  • WB analysis using 29898-1-AP (same clone as 29898-1-PBS)

    WB analysis using 29898-1-AP (same clone as 29898-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 29898-1-AP (NFIB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29898-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 29898-1-AP (same clone as 29898-1-PBS)

    IF Staining of HeLa using 29898-1-AP (same clone as 29898-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NFIB antibody (29898-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 29898-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 29898-1-AP (same clone as 29898-1-PBS)

    IF Staining of HepG2 using 29898-1-AP (same clone as 29898-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NFIB antibody (29898-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 29898-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 29898-1-AP (same clone as 29898-1-PBS)

    FC experiment of HeLa using 29898-1-AP (same clone as 29898-1-PBS)

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human NFIB (29898-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 29898-1-PBS in a different storage buffer formulation.

  • IHC staining of human skin cancer using 29898-1-AP (same clone as 29898-1-PBS)

    IHC staining of human skin cancer using 29898-1-AP (same clone as 29898-1-PBS)

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 29898-1-AP (NFIB antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29898-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 29898-1-AP (same clone as 29898-1-PBS)

    IF Staining of HeLa using 29898-1-AP (same clone as 29898-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NFIB antibody (29898-1-AP) at dilution of 1:1500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 29898-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

NFIB Polyclonal antibody, PBS Only

NFIB Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 29898-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

别名

CCAAT-box-binding transcription factor, CTF, HMGIC/NFIB, NF I/B, NF1 B

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

点击咨询售前客服

点击咨询售后客服

产品信息

29898-1-PBS targets NFIB in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag31946

Product name: Recombinant human NFIB protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 180-340 aa of BC001283

Sequence: LAYYVQEQDSGQSGSPSHNDPAKNPPGYLEDSFVKSGVFNVSELVRVSRTPITQGTGVNFPIGEIPSQPYYHDMNSGVNLQRSLSSPPSSKRPKTISIDENMEPSPTGDFYPSPSSPAAGSRTWHERDQDMSSPTTMKKPEKPLFSSASPQDSSPRLSTFP

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 nuclear factor I/B
别名 CCAAT-box-binding transcription factor, CTF, HMGIC/NFIB, NF I/B, NF1 B
计算分子量 47 kDa
观测分子量45-65 kDa
GenBank蛋白编号BC001283
基因名称 NFIB
Gene ID (NCBI) 4781
RRIDAB_3086183
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO00712
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

NFIB, also named as Nuclear factor 1, is a 420 amino acid protein, which belongs to the CTF/NF-I family and may bind DNA as a homodimer. NFIB recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication. NFIB also exists in multiple isoforms in prostate cancer cells, with average molecular weights of 62 kDa, 57 kDa, and 49 kDa (PMID: 32692871).

||
New chat

Able™

正在加载，请稍候...