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NFIB Polyclonal antibody
NFIB Polyclonal Antibody for FC, IF/ICC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human, mouse
Applications
FC, IF/ICC, WB, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | HUVEC cells, HeLa cells, HepG2 cells, NIH/3T3 cells |
Positive IF detected in | HepG2 cells |
Positive FC detected in | HeLa cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
29898-1-AP targets NFIB in WB, IF, FC, ELISA applications and shows reactivity with Human, mouse samples.
Tested Applications | FC, IF/ICC, WB, ELISA |
Tested Reactivity | Human, mouse |
Immunogen | NFIB fusion protein Ag31946 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | nuclear factor I/B |
Synonyms | CTF, HMGIC/NFIB, NF I/B, NF1 B, NFI B, NFI RED, NFIB, NFIB2, NFIB3, Nuclear factor 1 B type, Nuclear factor 1/B, nuclear factor I/B, TGGCA binding protein |
Calculated Molecular Weight | 47 kDa |
Observed Molecular Weight | 45-65 kDa |
GenBank Accession Number | BC001283 |
Gene Symbol | NFIB |
Gene ID (NCBI) | 4781 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | O00712 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for NFIB antibody 29898-1-AP | Download protocol |
IF protocol for NFIB antibody 29898-1-AP | Download protocol |
FC protocol for NFIB antibody 29898-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |