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验证数据展示

  • WB analysis using 21713-1-AP (same clone as 21713-1-PBS)

    WB analysis using 21713-1-AP (same clone as 21713-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 21713-1-AP (NFAT5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21713-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse skeletal muscle using 21713-1-AP (same clone as 21713-1-PBS)

    IHC staining of mouse skeletal muscle using 21713-1-AP (same clone as 21713-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 21713-1-AP (NFAT5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21713-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NFAT5 antibody included
  • IHC staining of mouse skeletal muscle using 21713-1-AP (same clone as 21713-1-PBS)

    IHC staining of mouse skeletal muscle using 21713-1-AP (same clone as 21713-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 21713-1-AP (NFAT5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21713-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NFAT5 antibody included
  • IF Staining of HepG2 using 21713-1-AP (same clone as 21713-1-PBS)

    IF Staining of HepG2 using 21713-1-AP (same clone as 21713-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NFAT5 antibody (21713-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red). This data was developed using the same antibody clone with 21713-1-PBS in a different storage buffer formulation.

NFAT5 Polyclonal antibody, PBS Only

NFAT5 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 21713-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat, monkey

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy NFAT5 Ready-To-Use IHC Kit. NFAT5 primary antibody included.

产品信息

21713-1-PBS targets NFAT5 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat, monkey samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat, monkey
免疫原

CatNo: Ag16341

Product name: Recombinant human NFAT5 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1231-1531 aa of BC146765

Sequence: MFQSQHSIVAMQSNSPSQEQQQQQQQQQQQQQQQQQSILFSNQNTMATMASPKQPPPNMIFNPNQNPMANQEQQNQSIFHQQSNMAPMNQEQQPMQFQSQSTVSSLQNPGPTQSESSQTPLFHSSPQIQLVQGSPSSQEQQVTLFLSPASMSALQTSINQQDMQQSPLYSPQNNMPGIQGATSSPQPQATLFHNTAGGTMNQLQNSPGSSQQTSGMFLFGIQNNCSQLLTSGPATLPDQLMAISQPGQPQNEGQPPVTTLLSQQMPENSPLASSINTNQNIEKIDLLVSLQNQGNNLTGSF

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 nuclear factor of activated T-cells 5, tonicity-responsive
别名
计算分子量 1531 aa, 166 kDa
观测分子量200 kDa
GenBank蛋白编号BC146765
基因名称 NFAT5
Gene ID (NCBI) 10725
RRIDAB_3085667
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO94916
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

NFAT5 (Nuclear factor of activated T-cells 5) is the member of the Rel family of transcription factors, including NF-κB and NFAT1-4, which plays central roles in inducible gene expression during the immune response (PMID: 21629436). NFAT5 also acts as a novel regulator of mammalian anti-pathogen responses, regulating TLR-induced gene expression in primary macrophages induced by Toll-like receptors (PMID: 22312110). NFAT5 is involved in the transcriptional regulation of osmoprotective and inflammatory genes (PMID: 10377394).

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