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验证数据展示

  • WB analysis using 21713-1-AP

    WB analysis using 21713-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21713-1-AP (NFAT5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse skeletal muscle using 21713-1-AP

    IHC staining of mouse skeletal muscle using 21713-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 21713-1-AP (NFAT5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with NFAT5 antibody included
  • IHC staining of mouse skeletal muscle using 21713-1-AP

    IHC staining of mouse skeletal muscle using 21713-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 21713-1-AP (NFAT5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with NFAT5 antibody included
  • IF Staining of HepG2 using 21713-1-AP

    IF Staining of HepG2 using 21713-1-AP

    Immunofluorescent analysis of HepG2 cells using 21713-1-AP (NFAT5 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

NFAT5 Polyclonal antibody

NFAT5 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 21713-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat, monkey

应用

WB, IHC, IF/ICC, ELISA

别名

Nuclear factor of activated T-cells 5, NFATZ, NFATL1, NF-AT5, NF AT5

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, Jurkat cells, U2OS cells, COS-7 cells
Positive IHC detected inmouse skeletal muscle tissue, human urothelial carcinoma tissue, human thyroid cancer tissue, mouse brain tissue, rat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy NFAT5 Ready-To-Use IHC Kit. NFAT5 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21713-1-AP targets NFAT5 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, monkey samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat, monkey
免疫原

CatNo: Ag16341

Product name: Recombinant human NFAT5 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1231-1531 aa of BC146765

Sequence: MFQSQHSIVAMQSNSPSQEQQQQQQQQQQQQQQQQQSILFSNQNTMATMASPKQPPPNMIFNPNQNPMANQEQQNQSIFHQQSNMAPMNQEQQPMQFQSQSTVSSLQNPGPTQSESSQTPLFHSSPQIQLVQGSPSSQEQQVTLFLSPASMSALQTSINQQDMQQSPLYSPQNNMPGIQGATSSPQPQATLFHNTAGGTMNQLQNSPGSSQQTSGMFLFGIQNNCSQLLTSGPATLPDQLMAISQPGQPQNEGQPPVTTLLSQQMPENSPLASSINTNQNIEKIDLLVSLQNQGNNLTGSF

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 nuclear factor of activated T-cells 5, tonicity-responsive
别名 Nuclear factor of activated T-cells 5, NFATZ, NFATL1, NF-AT5, NF AT5
计算分子量 1531 aa, 166 kDa
观测分子量200 kDa
GenBank蛋白编号BC146765
基因名称 NFAT5
Gene ID (NCBI) 10725
RRIDAB_3085667
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO94916
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NFAT5 (Nuclear factor of activated T-cells 5) is the member of the Rel family of transcription factors, including NF-κB and NFAT1-4, which plays central roles in inducible gene expression during the immune response (PMID: 21629436). NFAT5 also acts as a novel regulator of mammalian anti-pathogen responses, regulating TLR-induced gene expression in primary macrophages induced by Toll-like receptors (PMID: 22312110). NFAT5 is involved in the transcriptional regulation of osmoprotective and inflammatory genes (PMID: 10377394).

实验方案

Product Specific Protocols
IF protocol for NFAT5 antibody 21713-1-APDownload protocol
IHC protocol for NFAT5 antibody 21713-1-APDownload protocol
WB protocol for NFAT5 antibody 21713-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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