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验证数据展示

  • IF Staining of HepG2 using CL488-67685

    IF Staining of HepG2 using CL488-67685

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 NF45 antibody (CL488-67685, Clone: 1A3A2 ) at dilution of 1:200.

  • FC experiment of HepG2 using CL488-67685

    FC experiment of HepG2 using CL488-67685

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human NF45 (CL488-67685, Clone:1A3A2) (red), or 0.4 ug Mouse IgG2b Isotype Control (CL488-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

CoraLite® Plus 488-conjugated NF45 Monoclonal antibody

NF45 Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-67685

产品说明书

CloneNo. 1A3A2

宿主/亚型

Mouse / IgG2b

种属反应性

human, mouse, rat

应用

IF/ICC, FC (Intra)

别名

ILF2, PRO3063, Nuclear factor of activated T-cells 45 kDa, Interleukin enhancer-binding factor 2, 1A3A2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 67685-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67685 targets NF45 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原 NF45 fusion protein Ag30388 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 interleukin enhancer binding factor 2, 45kDa
别名 ILF2, PRO3063, Nuclear factor of activated T-cells 45 kDa, Interleukin enhancer-binding factor 2, 1A3A2
计算分子量 43 kDa
观测分子量40 kDa
GenBank蛋白编号BC000382
基因名称 ILF2
Gene ID (NCBI) 3608
RRIDAB_2919519
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ12905
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Interleukin-2 enhancer binding factor 2 (ILF2), also named as nuclear factor 45 (NF45), plays important roles in regulating interleukin-2 expression in mammals. It forms a heterodimeric complex with ILF3, which may regulate transcription of the IL2 gene during T-cell activation. It can also promote the formation of stable DNA-dependent protein kinase holoenzyme complexes on DNA [PMID:11739746,17129403].

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 NF45 antibody CL488-67685Download protocol
FC protocol for CL Plus 488 NF45 antibody CL488-67685Download protocol
Standard Protocols
Click here to view our Standard Protocols