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验证数据展示

  • WB analysis using 21686-1-AP (same clone as 21686-1-PBS)

    WB analysis using 21686-1-AP (same clone as 21686-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 21686-1-AP (NF2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21686-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 21686-1-AP (same clone as 21686-1-PBS)

    IHC staining of mouse brain using 21686-1-AP (same clone as 21686-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21686-1-AP (NF2 antibody) at dilution of 1:1500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21686-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NF2 antibody included
  • IP experiment of HeLa using 21686-1-AP (same clone as 21686-1-PBS)

    IP experiment of HeLa using 21686-1-AP (same clone as 21686-1-PBS)

    IP result of anti-NF2 (IP:21686-1-AP, 4ug; Detection:21686-1-AP 1:5000) with HeLa cells lysate 1560 ug. This data was developed using the same antibody clone with 21686-1-PBS in a different storage buffer formulation.

  • IHC staining of human meningioma using 21686-1-AP (same clone as 21686-1-PBS)

    IHC staining of human meningioma using 21686-1-AP (same clone as 21686-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human meningioma tissue slide using 21686-1-AP (NF2 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 21686-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NF2 antibody included
  • IHC staining of human meningioma using 21686-1-AP (same clone as 21686-1-PBS)

    IHC staining of human meningioma using 21686-1-AP (same clone as 21686-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human meningioma tissue slide using 21686-1-AP (NF2 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 21686-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NF2 antibody included
  • IHC staining of mouse brain using 21686-1-AP (same clone as 21686-1-PBS)

    IHC staining of mouse brain using 21686-1-AP (same clone as 21686-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21686-1-AP (NF2 antibody) at dilution of 1:1500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21686-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NF2 antibody included
  • IF Staining of HeLa using 21686-1-AP (same clone as 21686-1-PBS)

    IF Staining of HeLa using 21686-1-AP (same clone as 21686-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using NF2 antibody (21686-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 21686-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

Neurofibromin 2 Polyclonal antibody, PBS Only

Neurofibromin 2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 21686-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat, canine

应用

WB, IHC, IF/ICC, IP, ELISA

别名

NF2, Merlin, Moesin-ezrin-radixin-like protein, Neurofibromin-2

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy NF2 Ready-To-Use IHC Kit. NF2 primary antibody included.

产品信息

21686-1-PBS targets Neurofibromin 2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat, canine samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
经测试反应性 human, mouse, rat, canine
免疫原

CatNo: Ag16265

Product name: Recombinant human NF2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 245-595 aa of BC020257

Sequence: DPENRLTPKISFPWNEIRNISYSDKEFTIKPLDKKIDVFKFNSSKLRVNKLILQLCIGNHDLFMRRRKADSLEVQQMKAQAREEKARKQMERQRLAREKQMREEAERTRDELERRLLQMKEEATMANEALMRSEETADLLAEKAQITEEEAKLLAQKAAEAEQEMQRIKATAIRTEEEKRLMEQKVLEAEVLALKMAEESERRAKEADQLKQDLQEAREAERRAKQKLLEIATKPTYPPMNPIPAPLPPDIPSFNLIGDSLSFDFKDTDMKRLSMEIEKEKVEYMEKSKHLQEQLNELKTEIEALKLKERETALDILHNENSDRGGSSKHNTIKKLTLQSAKSRVAFFEEL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 neurofibromin 2 (merlin)
别名 NF2, Merlin, Moesin-ezrin-radixin-like protein, Neurofibromin-2
计算分子量 595 aa, 70 kDa
观测分子量 66-70 kDa
GenBank蛋白编号BC020257
基因名称 NF2
Gene ID (NCBI) 4771
RRIDAB_10792410
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP35240
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Neurofibromatosis 2 (NF2) tumor suppressor protein-merlin is involved in Hippo/SWH signaling pathway that associated with tumor suppression by restricting proliferation and promoting apoptosis. It has been reported that merlin can change to phosphorylation form which inactivates merlin tumor suppressive abilities at serine 518 induced by Rac effector p21-activated kinase 2. The mutation of the NF2 tumor suppressor gene may lead to nervous system tumors, mesotheliomas and metastatic tumors.

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