×
Use Able AI chat for product recommendations

验证数据展示

  • WB analysis using 87694-1-RR

    WB analysis using 87694-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 87694-1-RR (NF-L antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 87694-1-RR

    IHC staining of mouse brain using 87694-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 87694-1-RR (NF-L antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with NF-L antibody included
  • IHC staining of rat brain using 87694-1-RR

    IHC staining of rat brain using 87694-1-RR

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 87694-1-RR (NF-L antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with NF-L antibody included
  • IF Staining of mouse brain using 87694-1-RR

    IF Staining of mouse brain using 87694-1-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using NF-L antibody (87694-1-RR, Clone: 253172B6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse brain using 87694-1-RR

    IF Staining of mouse brain using 87694-1-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using NF-L antibody (87694-1-RR, Clone: 253172B6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity of 87694-1-RR

    Affinity of 87694-1-RR

    Biolayer interferometry (BLI) kinetic assays of 87694-1-RR against Human NF-L were performed. The affinity constant is 0.12 nM.

NF-L Recombinant monoclonal antibody

NF-L Uni-rAb® Recombinant Antibody for WB, IHC, IF-P, ELISA
Cat No. 87694-1-RR

产品说明书

CloneNo. 253172B6

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF-P, ELISA

别名

NEFL, neurofilament, NF68, NFL

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inHEK-293 cells, SH-SY5Y cells
Positive IHC detected inrat brain tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse brain tissue
Planning an IHC experiment? We recommend our IHCeasy NF-L Ready-To-Use IHC Kit. NF-L primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

87694-1-RR targets NF-L in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag3667

Product name: Recombinant human NEFL protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-352 aa of BC039237

Sequence: MSSFSYEPYYSTSYKRRYVETPRVHISSVRSGYSTARSAYSSYSAPVSSSLSVRRSYSSSSGSLMPSLENLDLSQVAAISNDLKSIRTQEKAQLQDLNDRFASFIERVHELEQQNKVLEAELLVLRQKHSEPSRFRALYEQEIRDLRLAAEDATNEKQALQGEREGLEETLRNLQARYEEEVLSREDAEGRLMEARKGADEAALARAELEKRIDSLMDEISFLKKVHEEEIAELQAQIQYAQISVEMDVTKPDLSAALKDIRAQYEKLAAKNMQNAEEWFKSRFTVLTESAAKNTDAVRAAKDEVSESRRLLKAKTLEIEACRGMNEALEKQLQELEDKQNADISAMQDTIN

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 neurofilament, light polypeptide
别名 NEFL, neurofilament, NF68, NFL
计算分子量 543 aa, 62 kDa
观测分子量 65 kDa
GenBank蛋白编号BC039237
基因名称 NF-L
Gene ID (NCBI) 4747
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP07196
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NEFL, also named as NF68 and NF-L, belongs to the intermediate filament family. Neurofilaments are the 10 nm intermediate filaments found specifically in neurons. They are a major component of the cell's cytoskeleton, and provide support for normal axonal radial growth. Neurofilaments usually contain three intermediate filament proteins: L, M, and H, which are involved in the maintenance of neuronal caliber. The names given to the three major neurofilament subunits are based upon the apparent molecular weight of the mammalian subunits on SDS-PAGE: NF-L, 65-68 kDa; NF-M, 145-160 kDa and NF-H, 200-220 kDa.

实验方案

Product Specific Protocols
IF protocol for NF-L antibody 87694-1-RRDownload protocol
IHC protocol for NF-L antibody 87694-1-RRDownload protocol
WB protocol for NF-L antibody 87694-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
||
New chat

Able™

正在加载，请稍候...