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验证数据展示

  • WB analysis using 87694-1-RR (same clone as 87694-1-PBS)

    WB analysis using 87694-1-RR (same clone as 87694-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 87694-1-RR (NF-L antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87694-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 87694-1-RR (same clone as 87694-1-PBS)

    IHC staining of mouse brain using 87694-1-RR (same clone as 87694-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 87694-1-RR (NF-L antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 87694-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NF-L antibody included
  • IHC staining of rat brain using 87694-1-RR (same clone as 87694-1-PBS)

    IHC staining of rat brain using 87694-1-RR (same clone as 87694-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 87694-1-RR (NF-L antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 87694-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NF-L antibody included
  • IF Staining of mouse brain using 87694-1-RR (same clone as 87694-1-PBS)

    IF Staining of mouse brain using 87694-1-RR (same clone as 87694-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using NF-L antibody (87694-1-RR, Clone: 253172B6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 87694-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse brain using 87694-1-RR (same clone as 87694-1-PBS)

    IF Staining of mouse brain using 87694-1-RR (same clone as 87694-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using NF-L antibody (87694-1-RR, Clone: 253172B6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 87694-1-PBS in a different storage buffer formulation.

  • Cytometric bead array sample test of MP03161-1

    Cytometric bead array sample test of MP03161-1

    The mean NF-L concentration was determined to be 3685.4 ng/mL in mouse brain tissue extract based on a 6.3 mg/mL extract load, 4.9 ng/mL in mouse liver tissue extract based on a 8.4 mg/mL extract load.

  • Cytometric bead array standard curve of MP03161-1

    Cytometric bead array standard curve of MP03161-1

    Cytometric bead array standard curve of MP03161-1, NF-L Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 87694-3-PBS. Detection antibody: 87694-1-PBS. Standard: Ag3667. Range: 0.312-40 ng/mL.

  • Affinity of 87694-1-RR

    Affinity of 87694-1-RR

    Biolayer interferometry (BLI) kinetic assays of 87694-1-RR against Human NF-L were performed. The affinity constant is 0.12 nM.

NF-L Recombinant monoclonal antibody, PBS Only (Detector)

NF-L Uni-rAb® Recombinant Antibody for WB, IHC, IF-P, Cytometric bead array, Indirect ELISA
Cat No. 87694-1-PBS

产品说明书

CloneNo. 253172B6

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF-P, Cytometric bead array, Indirect ELISA

别名

NEFL, neurofilament, NF68, NFL

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy NF-L Ready-To-Use IHC Kit. NF-L primary antibody included.

产品信息

87694-1-PBS targets NF-L as part of a matched antibody pair:

MP03161-1: 87694-3-PBS capture and 87694-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, IF-P, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag3667

Product name: Recombinant human NEFL protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-352 aa of BC039237

Sequence: MSSFSYEPYYSTSYKRRYVETPRVHISSVRSGYSTARSAYSSYSAPVSSSLSVRRSYSSSSGSLMPSLENLDLSQVAAISNDLKSIRTQEKAQLQDLNDRFASFIERVHELEQQNKVLEAELLVLRQKHSEPSRFRALYEQEIRDLRLAAEDATNEKQALQGEREGLEETLRNLQARYEEEVLSREDAEGRLMEARKGADEAALARAELEKRIDSLMDEISFLKKVHEEEIAELQAQIQYAQISVEMDVTKPDLSAALKDIRAQYEKLAAKNMQNAEEWFKSRFTVLTESAAKNTDAVRAAKDEVSESRRLLKAKTLEIEACRGMNEALEKQLQELEDKQNADISAMQDTIN

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 neurofilament, light polypeptide
别名 NEFL, neurofilament, NF68, NFL
计算分子量 543 aa, 62 kDa
观测分子量 65 kDa
GenBank蛋白编号BC039237
基因名称 NF-L
Gene ID (NCBI) 4747
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP07196
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

NEFL, also named as NF68 and NF-L, belongs to the intermediate filament family. Neurofilaments are the 10 nm intermediate filaments found specifically in neurons. They are a major component of the cell's cytoskeleton, and provide support for normal axonal radial growth. Neurofilaments usually contain three intermediate filament proteins: L, M, and H, which are involved in the maintenance of neuronal caliber. The names given to the three major neurofilament subunits are based upon the apparent molecular weight of the mammalian subunits on SDS-PAGE: NF-L, 65-68 kDa; NF-M, 145-160 kDa and NF-H, 200-220 kDa.

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