验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells, Raji cells, Ramos cells, SH-SY5Y cells, SK-N-SH cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
33450-1-AP targets NEPRO in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag38276 Product name: Recombinant human C3orf17 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-168 aa of NM_015412.3 Sequence: MMAAVPPGLEPWNRVRIPKAGNRSAVTVQNPGAALDLCIAAVIKECHLVILSLKSQTLDAETDVLCAVLYSNHNRMGRHKPHLALKQVEQCLKRLKNMNLEGSIQDLFELFSSNENQPLTTKVCVVPSQPVVELVLMKVLGACKLLLRLLDCCCKTFLLTVKHLGLQE 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | chromosome 3 open reading frame 17 |
| 别名 | C3orf17, Nucleolus and neural progenitor protein |
| 计算分子量 | 65kDa，567aa |
| 观测分子量 | 58 kDa |
| GenBank蛋白编号 | NM_015412.3 |
| 基因名称 | C3orf17 |
| Gene ID (NCBI) | 25871 |
| RRID | AB_3742976 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | Q6NW34 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The NEPRO protein (full name: Nucleolus and Neural Progenitor Protein) belongs to the NEPRO family and is primarily localized in the nucleolus. Its core function is to serve as a key downstream factor of the Notch signaling pathway during cerebral cortex development, where it maintains the stemness of neural progenitor cells by inhibiting their differentiation into neurons, thereby ensuring normal development of the nervous system. Additionally, this protein also plays an indispensable role during pre-implantation embryonic development in mice (PMID: 26178919).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for NEPRO antibody 33450-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
