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验证数据展示

  • WB analysis using 15760-1-AP

    WB analysis using 15760-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15760-1-AP (Neudesin/NENF antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Neudesin/NENF Polyclonal antibody

Neudesin/NENF Polyclonal Antibody for WB, ELISA
Cat No. 15760-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

Neudesin / NENF, Neudesin, NENF, Protein GIG47, Neuron-derived neurotrophic factor

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHCT 116 cells, HEK-293 cells, HeLa cells, MCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy NENF Ready-To-Use IHC Kit. NENF primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15760-1-AP targets Neudesin/NENF in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag8387

Product name: Recombinant human NENF protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 33-172 aa of BC008823

Sequence: QTPRPAERGPPVRLFTEEELARYGGEEEDQPIYLAVKGVVFDVTSGKEFYGRGAPYNALTGKDSTRGVAKMSLDPADLTHDTTGLTAKELEALDEVFTKVYKAKYPIVGYTARRILNEDGSPNLDFKPEDQPHFDIKDEF

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 neuron derived neurotrophic factor
别名 Neudesin / NENF, Neudesin, NENF, Protein GIG47, Neuron-derived neurotrophic factor
计算分子量 172 aa, 19 kDa
观测分子量19 kDa
GenBank蛋白编号BC008823
基因名称 NENF
Gene ID (NCBI) 29937
RRIDAB_2150989
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UMX5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Neudesin neurotrophic factor (NENF, also known as CIR2, SPUF, and SCIRP10) acts as a neurotrophic factor in postnatal mature neurons enhancing neuronal survival, which is localized to mitochondria and endoplasmic reticulum by PINK1 and PARK7 (PMID: 31536960). NENF in the adult brain is expected to play roles in the maintenance and protection of neurons in an autocrine/paracrine manner (PMID: 15605373). It greatly increases cAMP levels in neural precursor cells and might activate a Gs-protein-coupled receptor that could activate the MAPK, PKA, and PI-3K signal pathways (PMID: 16547973).

实验方案

Product Specific Protocols
WB protocol for Neudesin/NENF antibody 15760-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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