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验证数据展示

  • WB analysis of HeLa using 10456-1-AP (same clone as 10456-1-PBS)

    WB analysis of HeLa using 10456-1-AP (same clone as 10456-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 10456-1-AP (NELF-A Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10456-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse brain using 10456-1-AP (same clone as 10456-1-PBS)

    IP experiment of mouse brain using 10456-1-AP (same clone as 10456-1-PBS)

    IP result of anti-NELF-A (IP:10456-1-AP, 4ug; Detection:10456-1-AP 1:500) with mouse brain tissue lysate 3440ug. This data was developed using the same antibody clone with 10456-1-PBS in a different storage buffer formulation.

  • WB analysis using 10456-1-AP (same clone as 10456-1-PBS)

    WB analysis using 10456-1-AP (same clone as 10456-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 10456-1-AP (NELF-A antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10456-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 10456-1-AP (same clone as 10456-1-PBS)

    IF Staining of HeLa using 10456-1-AP (same clone as 10456-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NELF-A antibody (10456-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 10456-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

NELF-A Polyclonal antibody, PBS Only

NELF-A Polyclonal Antibody for WB, IF/ICC, IP, Indirect ELISA
Cat No. 10456-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, IP, Indirect ELISA

别名

WHSC2, Wolf-Hirschhorn syndrome candidate 2 protein, NELFA, NELF A

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

10456-1-PBS targets NELF-A in WB, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag0708

Product name: Recombinant human NELF-A protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 6-314 aa of BC002764

Sequence: ESDTGLWLHNKLGATDELWAPPSIASLLTAAVIDNIRLCFHGLSSAVKLKLLLGTLHLPRRTVDEMKGALMEIIQLASLDSDPWVLMVADILKSFPDTGSLNLELEEQNPNVQDILGELREKVGECEASAMLPLECQYLNKNALTTLAGPLTPPVKHFQLKRKPKSATLRAELLQKSTETAQQLKRSAGVPFHAKGRGLLRKMDTTTPLKGIPKQAPFRSPTAPSVFSPTGNRTPIPPSRTLLRKERGVKLLDISELDMVGAGREAKRRRKTLDAEVVEKPAKEETVVENATPDYAAGLVSTQKLGSLN

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 Wolf-Hirschhorn syndrome candidate 2
别名 WHSC2, Wolf-Hirschhorn syndrome candidate 2 protein, NELFA, NELF A
计算分子量 57 kDa
观测分子量 57 kDa
GenBank蛋白编号BC002764
基因名称 NELFA
Gene ID (NCBI) 7469
RRIDAB_2216327
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9H3P2
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

WHSC2, also known as NELF-A, is a component of the Negative ELongation Factor complex, which inhibits RNA polymerase II progression early during elongation (a process referred to as 'promoter proximal pausing'), thereby altering expression of its target genes in a positive and negative manner [PMID:19245807]. This complex acts with DRB sensitivity-inducing factor (DSIF), a heterodimer of SPT4 and SPT5, to cause transcriptional pausing of RNA polymerase II[PMID:12612062].

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