- Featured Product
- KD/KO Validated
NELF-A Polyclonal antibody
NELF-A Polyclonal Antibody for IF/ICC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
IF/ICC, IP, WB, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, mouse liver tissue, Jurkat cells, mouse brain tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IF detected in | HeLa cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF) | IF : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 2 publications below |
| WB | See 4 publications below |
产品信息
10456-1-AP targets NELF-A in WB, IP, IF, ELISA applications and shows reactivity with human, mouse samples.
| Tested Applications | IF/ICC, IP, WB, ELISA |
| Cited Applications | WB |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Immunogen | NELF-A fusion protein Ag0708 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | Wolf-Hirschhorn syndrome candidate 2 |
| Synonyms | Negative elongation factor A, NELF A, NELFA, NELF-A, P/OKcl.15, WHSC2 |
| Calculated Molecular Weight | 57 kDa |
| Observed Molecular Weight | 57 kDa |
| GenBank Accession Number | BC002764 |
| Gene Symbol | WHSC2 |
| Gene ID (NCBI) | 7469 |
| RRID | AB_2216327 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9H3P2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
WHSC2, also known as NELF-A, is a component of the Negative ELongation Factor complex, which inhibits RNA polymerase II progression early during elongation (a process referred to as 'promoter proximal pausing'), thereby altering expression of its target genes in a positive and negative manner [PMID:19245807]. This complex acts with DRB sensitivity-inducing factor (DSIF), a heterodimer of SPT4 and SPT5, to cause transcriptional pausing of RNA polymerase II[PMID:12612062].
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for NELF-A antibody 10456-1-AP | Download protocol |
| IF protocol for NELF-A antibody 10456-1-AP | Download protocol |
| IP protocol for NELF-A antibody 10456-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun RNA polymerase II pausing factor NELF in CD8+ T cells promotes antitumor immunity. | ||
Nucleic Acids Res Multiple P-TEFbs cooperatively regulate the release of promoter-proximally paused RNA polymerase II.
| ||
Nat Commun ERK-mediated NELF-A phosphorylation promotes transcription elongation of immediate-early genes by releasing promoter-proximal pausing of RNA polymerase II
| ||
J Biol Chem An Essential Signaling Function of Cytoplasmic NELFB is Independent of RNA Polymerase II Pausing |
