×
Use Able AI chat for product recommendations

验证数据展示

  • WB analysis of HeLa using 27146-1-AP

    WB analysis of HeLa using 27146-1-AP

    WB result of NEK1 antibody (27146-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NEK1 transfected HeLa cells.

  • WB analysis of PC-12 using 27146-1-AP

    WB analysis of PC-12 using 27146-1-AP

    PC-12 cells were subjected to SDS PAGE followed by western blot with 27146-1-AP (NEK1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IP experiment of PC-12 using 27146-1-AP

    IP experiment of PC-12 using 27146-1-AP

    IP result of anti-NEK1 (IP:27146-1-AP, 4ug; Detection:27146-1-AP 1:500) with PC-12 cells lysate 3080 ug.

  • IP experiment of HeLa using 27146-1-AP

    IP experiment of HeLa using 27146-1-AP

    HeLa lysates prepared and IP of NEK1 performed using 1.0 μg of 27146-1-AP coupled to protein A- Sepharose beads. The Ponceau stained transfers of each blot are shown. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency.

  • IHC staining of human lung cancer using 27146-1-AP

    IHC staining of human lung cancer using 27146-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 27146-1-AP (NEK1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with NEK1 antibody included
  • IHC staining of human lung cancer using 27146-1-AP

    IHC staining of human lung cancer using 27146-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 27146-1-AP (NEK1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with NEK1 antibody included
  • IHC staining of mouse kidney using 27146-1-AP

    IHC staining of mouse kidney using 27146-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 27146-1-AP (NEK1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with NEK1 antibody included
  • IHC staining of human appendicitis using 27146-1-AP

    IHC staining of human appendicitis using 27146-1-AP

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 27146-1-AP (NEK1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with NEK1 antibody included
  • IHC staining of human stomach cancer using 27146-1-AP

    IHC staining of human stomach cancer using 27146-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 27146-1-AP (NEK1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with NEK1 antibody included
  • IHC staining of mouse stomach using 27146-1-AP

    IHC staining of mouse stomach using 27146-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 27146-1-AP (NEK1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with NEK1 antibody included
  • IHC staining of rat kidney using 27146-1-AP

    IHC staining of rat kidney using 27146-1-AP

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 27146-1-AP (NEK1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with NEK1 antibody included
  • IHC staining of rat stomach using 27146-1-AP

    IHC staining of rat stomach using 27146-1-AP

    Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 27146-1-AP (NEK1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with NEK1 antibody included
  • IHC staining of mouse stomach using 27146-1-AP

    IHC staining of mouse stomach using 27146-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 27146-1-AP (NEK1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with NEK1 antibody included
  • IHC staining of rat stomach using 27146-1-AP

    IHC staining of rat stomach using 27146-1-AP

    Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 27146-1-AP (NEK1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with NEK1 antibody included
  • IF Staining of HeLa using 27146-1-AP

    IF Staining of HeLa using 27146-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using NEK1 antibody (27146-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Featured Product
  • KD/KO Validated

NEK1 Polyclonal antibody

NEK1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 27146-1-AP

产品说明书

引用文献(4)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, ELISA and More (1)

别名

EC:2.7.11.1, KIAA1901, Never in mitosis A-related kinase 1, NimA related protein kinase 1, NimA-related protein kinase 1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inPC-12 cells, mouse pancreas tissue, HeLa cells
Positive IP detected inPC-12 cells, HeLa cells
Positive IHC detected inhuman lung cancer tissue, rat stomach tissue, mouse stomach tissue, mouse kidney tissue, rat kidney tissue, human appendicitis tissue, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy NEK1 Ready-To-Use IHC Kit. NEK1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27146-1-AP targets NEK1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB, IHC, IP, CoIP
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原

CatNo: Ag25970

Product name: Recombinant human NEK1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 557-740 aa of BC114491

Sequence: KRKEAYEREKKVWEEHLVAKGVKSSDVSPPLGQHETGGSPSKQQMRSVISVTSALKEVGVDSSLTDTRETSEEMQKTNNAISSKREILRRLNENLKAQEDEKGKQNLSDTFEINVHEDAKEHEKEKSVSSDRKKWEAGGQLVIPLDELTLDTSFSTTERHTVGEVIKLGPNGSPRRAWGKSPTD

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 NIMA (never in mitosis gene a)-related kinase 1
别名 EC:2.7.11.1, KIAA1901, Never in mitosis A-related kinase 1, NimA related protein kinase 1, NimA-related protein kinase 1
计算分子量 1258 aa, 143 kDa
观测分子量 170 kDa
GenBank蛋白编号BC114491
基因名称 NEK1
Gene ID (NCBI) 4750
RRIDAB_2880773
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96PY6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for NEK1 antibody 27146-1-APDownload protocol
IHC protocol for NEK1 antibody 27146-1-APDownload protocol
IP protocol for NEK1 antibody 27146-1-APDownload protocol
WB protocol for NEK1 antibody 27146-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Adv Sci (Weinh)

HDAC8 Enhances the Function of HIF-2α by Deacetylating ETS1 to Decrease the Sensitivity of TKIs in ccRCC

Authors - Kang Qian
  • KD Validated
View Article
humanWB,IHC,CoIP

Neoplasia

MTFR1 phosphorylation-activated adaptive mitochondrial fusion is essential for colon cancer cell survival during glucose deprivation

Authors - Nan Zhang
  • KD Validated
View Article
humanWB,IHC

Cancers (Basel)

Differential Expression of NEK Kinase Family Members in Esophageal Adenocarcinoma and Barrett's Esophagus

Authors - Lei Chen
View Article
humanWB,IP

Life Sci Alliance

Functional characterization of C21ORF2 association with the NEK1 kinase mutated in human in diseases

Authors - Mateusz Gregorczyk
View Article
||
New chat

Able™

正在加载，请稍候...