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验证数据展示

  • WB analysis of HeLa using 27146-1-AP

    WB analysis of HeLa using 27146-1-AP

    WB result of NEK1 antibody (27146-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NEK1 transfected HeLa cells.

  • WB analysis of PC-12 using 27146-1-AP

    WB analysis of PC-12 using 27146-1-AP

    PC-12 cells were subjected to SDS PAGE followed by western blot with 27146-1-AP (NEK1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IP experiment of PC-12 using 27146-1-AP

    IP experiment of PC-12 using 27146-1-AP

    IP result of anti-NEK1 (IP:27146-1-AP, 4ug; Detection:27146-1-AP 1:500) with PC-12 cells lysate 3080 ug.

  • IP experiment of HeLa using 27146-1-AP

    IP experiment of HeLa using 27146-1-AP

    HeLa lysates prepared and IP of NEK1 performed using 1.0 μg of 27146-1-AP coupled to protein A- Sepharose beads. The Ponceau stained transfers of each blot are shown. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency.

  • IHC staining of human lung cancer using 27146-1-AP

    IHC staining of human lung cancer using 27146-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 27146-1-AP (NEK1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with NEK1 antibody included
  • IHC staining of human lung cancer using 27146-1-AP

    IHC staining of human lung cancer using 27146-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 27146-1-AP (NEK1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with NEK1 antibody included
  • IF Staining of HeLa using 27146-1-AP

    IF Staining of HeLa using 27146-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using NEK1 antibody (27146-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Featured Product
  • KD/KO验证

NEK1 Polyclonal antibody

NEK1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 27146-1-AP

产品说明书

引用文献(3)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, ELISA

别名

EC:2.7.11.1, KIAA1901, Never in mitosis A-related kinase 1, NimA related protein kinase 1, NimA-related protein kinase 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inPC-12 cells, mouse pancreas tissue, HeLa cells
Positive IP detected inPC-12 cells, HeLa cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy NEK1 Ready-To-Use IHC Kit. NEK1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27146-1-AP targets NEK1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB, IHC, IP
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原

CatNo: Ag25970

Product name: Recombinant human NEK1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 557-740 aa of BC114491

Sequence: KRKEAYEREKKVWEEHLVAKGVKSSDVSPPLGQHETGGSPSKQQMRSVISVTSALKEVGVDSSLTDTRETSEEMQKTNNAISSKREILRRLNENLKAQEDEKGKQNLSDTFEINVHEDAKEHEKEKSVSSDRKKWEAGGQLVIPLDELTLDTSFSTTERHTVGEVIKLGPNGSPRRAWGKSPTD

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 NIMA (never in mitosis gene a)-related kinase 1
别名 EC:2.7.11.1, KIAA1901, Never in mitosis A-related kinase 1, NimA related protein kinase 1, NimA-related protein kinase 1
计算分子量 1258 aa, 143 kDa
观测分子量 170 kDa
GenBank蛋白编号BC114491
基因名称 NEK1
Gene ID (NCBI) 4750
RRIDAB_2880773
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96PY6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for NEK1 antibody 27146-1-APDownload protocol
IHC protocol for NEK1 antibody 27146-1-APDownload protocol
IF protocol for NEK1 antibody 27146-1-APDownload protocol
IP protocol for NEK1 antibody 27146-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IP

Life Sci Alliance

Functional characterization of C21ORF2 association with the NEK1 kinase mutated in human in diseases

Authors - Mateusz Gregorczyk
View Article
humanWB,IHC

Cancers (Basel)

Differential Expression of NEK Kinase Family Members in Esophageal Adenocarcinoma and Barrett's Esophagus

Authors - Lei Chen
View Article
humanWB

Adv Sci (Weinh)

HDAC8 Enhances the Function of HIF-2α by Deacetylating ETS1 to Decrease the Sensitivity of TKIs in ccRCC

Authors - Kang Qian
  • KD Validated
View Article