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NEB-Specific Polyclonal antibody

NEB-Specific Polyclonal Antibody for IHC,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC,ELISA and More (1)

Conjugate

Unconjugated

Cat No : 19706-1-AP

Print datasheet

Synonyms

NEB, NEB177D, nebulin, NEM2

验证数据展示

  • IHC staining of human skeletal muscle using 19706-1-AP

    IHC staining of human skeletal muscle using 19706-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 19706-1-AP (NEB-Specific antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human skeletal muscle using 19706-1-AP

    IHC staining of human skeletal muscle using 19706-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 19706-1-AP (NEB-Specific antibody) at dilution of 1:50 (under 40x lens).

经过测试的应用

Positive IHC detected inhuman skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

19706-1-AP targets NEB-Specific in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC,ELISA
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name nebulin
Synonyms NEB, NEB177D, nebulin, NEM2
Calculated Molecular Weight 773 kDa
GenBank Accession NumberNM_004543
Gene Symbol NEB
Gene ID (NCBI) 4703
RRIDAB_10637999
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP20929
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NEB, a giant muscle protein, may be involved in maintaining the structural integrity of sarcomeres and the membrane system associated with the myofibrils. NEB binds and stabilizes F-actin. Defects in NEB are the cause of nemaline myopathy type 2 (NEM2). The antibody is specific to NEB.

实验方案

Product Specific Protocols
IHC protocol for NEB-Specific antibody 19706-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Clin Invest

KLHL40 deficiency destabilizes thin filament proteins and promotes nemaline myopathy.

Authors - Ankit Garg
View Article
mouseWB

Elife

KLHL41 stabilizes skeletal muscle sarcomeres by nonproteolytic ubiquitination.

Authors - Andres Ramirez-Martinez
View Article