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验证数据展示

  • WB analysis using 87156-1-RR

    WB analysis using 87156-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 87156-1-RR (NDUFS8 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis using 87156-1-RR

    WB analysis using 87156-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 87156-1-RR (NDUFS8 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse heart using 87156-1-RR

    IHC staining of mouse heart using 87156-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 87156-1-RR (NDUFS8 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse heart using 87156-1-RR

    IHC staining of mouse heart using 87156-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 87156-1-RR (NDUFS8 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 87156-1-RR

    IF Staining of HeLa using 87156-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NDUFS8 antibody (87156-1-RR, Clone: 252267D5 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity of 87156-1-RR

    Affinity of 87156-1-RR

    Biolayer interferometry (BLl) kinetic assays of 87156-1-RR against Human NDUFS8 were performed. The affinity constant is below 1 pM.

NDUFS8 Recombinant monoclonal antibody

NDUFS8 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 87156-1-RR

产品说明书

CloneNo. 252267D5

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

CI 23kD, CI-23kD, Complex I 23kD, Complex I-23kD, EC:7.1.1.2

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, mouse heart tissue, HepG2 cells, Jurkat cells, rat heart tissue
Positive IHC detected inmouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

87156-1-RR targets NDUFS8 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag18531

Product name: Recombinant human NDUFS8 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-210 aa of BC119754

Sequence: MRCLTTPMLLRALAQAARAGPPGGRSLHSSAVAATYKYVNMQDPEMDMKSVTDRAARTLLWTELFRGLGMTLSYLFREPATINYPFEKGPLSPRFRGEHALRRYPSGEERCIACKLCEAICPAQAITIEAEPRADGSRRTTRYDIDMTKCIYCGFCQEACPVDAIVEGPNFEFSTETHEELLYNKEKLLNNGDKWEAEIAANIQADYLYR

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 NADH dehydrogenase (ubiquinone) Fe-S protein 8, 23kDa (NADH-coenzyme Q reductase)
别名 CI 23kD, CI-23kD, Complex I 23kD, Complex I-23kD, EC:7.1.1.2
计算分子量 210 aa, 24 kDa
观测分子量 24 kDa
GenBank蛋白编号BC119754
基因名称 NDUFS8
Gene ID (NCBI) 4728
RRIDAB_3745377
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDO00217
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NADH: ubiquinone oxidoreductase core subunit S8 (NDUFS8), also called TYKY, is an essential core subunit and component of the iron-sulfur (FeS) fragment of mitochondrial complex I directly involved in the electron transfer process and energy metabolism (PMID: 36557887). Pathogenic variants of the NDUFS8 are relevant to infantile-onset and severe diseases, including Leigh syndrome, cancer, and diabetes mellitus (PMID: 36557887). Knockdown of NDUFS8 impaired mitochondrial function, increased ROS, disrupted redox homeostasis, induced apoptosis, and suppressed proliferation and migration of HCC cells (PMID: 40914145).

实验方案

Product Specific Protocols
IF protocol for NDUFS8 antibody 87156-1-RRDownload protocol
IHC protocol for NDUFS8 antibody 87156-1-RRDownload protocol
WB protocol for NDUFS8 antibody 87156-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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